C57bl 6jolahsd bl6 mice

Female, 4-10 weeks old C57BL/6JOlaHsd (BL6) mice were purchased from Envigo RMS GmbH and were housed under pathogen-free conditions at the animal facility of the University of Veterinary Medicine Hannover, Foundation, in individually ventilated cages type Sealsafe Plus GM500 or IsoCage N Biocontainment system (Tecniplast) for the entire duration of the experiment. All mice were subjected to two weeks of habituation and acclimatization before they were taken into the experiments. Sterilized food pellets and water were provided ad libitum.

Six-week old female C57BL/6JOlaHsd (BL6) mice were obtained from Envigo, Inc. (Indianapolis, IN, USA). Mice were housed in isocage systems with individually ventilated cages. Experiments were done in biosafety level 3 laboratories of the Institute of Parasitology, Biology Center of Czech Academy of Sciences, České Budějovice, Czech Republic. The protocol was approved by the Departmental Expert Committee for the Approval of Projects of Experiments on Animals of the Czech Academy of Sciences and the Committee on the Ethics of Animal Experimentation at the Institute of Parasitology (permit No. 29/2016). All experiments were done in accordance with Czech national law guidelines (animal Welfare Act No. 246/1992 Col.) and European Union guidelines for work with animals.