At predetermined time points (07:00) on day 101, blood specimens were collected from the jugular veins of eight individuals per group into vacutainer tubes. Subsequently, samples underwent immediate centrifugation at 3000 rpm and 4 °C, with plasma being extracted and preserved at −20 °C until further analysis. Plasma physiology and biochemistry, encompassing total protein (TP), albumin (ALB), globulin (GLOB), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-CH), low-density lipoprotein cholesterol (LDL-CH), glucose (GLU), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT), were quantified employing an automated biochemistry analyzer (Roche, Basel, Switzerland).
Automated biochemistry analyzer
Manufactured by Roche
Sourced in Switzerland, Germany
The automated biochemistry analyzer is a laboratory instrument designed to perform automated analysis of various biochemical parameters in biological samples, such as blood or urine. The core function of this device is to provide efficient and accurate measurement of analytes, including enzymes, proteins, and other biomolecules, through a fully automated process.
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Lab products found in correlation
7 protocols using automated biochemistry analyzer
1
Plasma Biomarker Analysis Protocol
2
Comprehensive Clinical Assessments for NAFLD
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All patients received physical and biochemical examinations after admission to the hospital. Weight (without shoes and in light outdoor clothing) and height were measured. Body mass index (BMI, kg/m2) was calculated by dividing weight (kg) by height squared (m2). Blood pressure was measured with the Riva-Rocci sphygmomanometer. All venous blood samples were taken in the morning following an overnight fasting for at least 10 hours. Serum total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-cholesterol) and high-density lipoprotein cholesterol (HDL-cholesterol), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured using Beckman Biochemical Analyzer (DXC800, USA). HbA1c was measured by high performance liquid chromatography with an automated biochemistry analyzer (Roche, Switzerland). Urine acetone bodies were measured by chemical analysis. Hepatic ultrasonography scanning was performed on all subjects after an overnight fast by assigned and experienced radiologists who were blinded to subjects' details. Nonalcoholic fatty liver disease (NAFLD) was defined by liver ultrasonographic scanning and diagnosed according to the standard set by the Chinese Association of Medicine in 2010 [8 (link)], excluding viral hepatitis in nondrinkers.
3
Enzymatic Biomarker Analysis Protocol
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The fasting glucose levels were determined with an automatic enzymatic colorimetric method using hexokinase (Cobas Integra; Roche, Basel, Switzerland). The TC, HDLc, LDLc, VLDLc and TG levels were analyzed with an automatic enzymatic colorimetric method (Cobas Mira; F. Hoffmann-La Roche, Basel, Switzerland). AST, ALT and GGT were measured with an automated biochemistry analyzer (Roche, Mannheim, Germany).
4
Blood Serum Analysis Protocol
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The blood samples were immediately collected by removing the eyeball and centrifuged at 4000 × g for 10 min to collect the serum after it was kept at room temperature for one hour. The serum was isolated for AST, ALT, TG, and TC evaluation, which were measured by the automated biochemistry analyzer (Roche, Mannheim, Germany) and according to the manufacturer's instructions. Commercial enzymatic tests were performed for TG and TC determination, while velocity tests were used for AST and ALT detection.
5
Serum Biomarker Analysis Protocol
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After the collected blood sample kept at room temperature for one hour, the serum sample was obtained by centrifugation at 4000× g at 4 °C for 10 min. Subsequently, the activities of serum aspartate transaminase (AST) and alanine transaminase (ALT), and the levels of serum total cholesterol (TC) and triglyceride (TG) in each group were, respectively, determined by the automated biochemistry analyzer (Roche, Mannheim, Germany). In addition, the kits of serum ALT, AST, TG, and TC were purchased from Roche diagnostics (Shanghai, China).
6
Serum Biochemical Profiling Protocol
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Blood samples were collected and centrifuged at 4000× g for 15 min at 4 °C to obtain serum, which was stored at 4 °C for further biochemical testing. Kits for serum triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were obtained from Roche Diagnostics (Shanghai, China). Additionally, the concentrations of serum TG, TC, and LDL-C, as well as the activities of serum ALT and AST, were measured with an automated biochemistry analyzer (Roche, Mannheim, Germany).
7
Liver and Lipid Biomarker Profiling
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The liver function biomarkers of alanine transaminase (ALT) and aspartate transaminase (AST) as well as the serum lipid profile of triglyceride (TG) and total cholesterol (TC) were determined with the corresponding kits by using an automated biochemistry analyzer (Roche Diagnostics GmbH, Mannheim, Germany).
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