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Tc 2000 meter

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The TC-2000 Meter is a device used for measuring temperature and conductivity. It provides accurate and reliable measurements of these parameters.

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Lab products found in correlation

Th 5 thermometer, by Physitemp (8 mentions) It 18, by Physitemp (1 mentions)

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TC-2000 (12 citations)

10 protocols using tc 2000 meter

1

Tissue Temperature Monitoring in Mice

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For tissue temperature recording, type T thermocouple probes and/or fiber optic temperature probe were implanted into the adipose tissue of mice under anesthesia (Urethane at 1.3 g kg−1). Device implantation was performed according to the procedures described in previous studies10 (link),40 (link). Tissue temperature was recorded by TC-2000 Meter (Sable Systems International) or Reflex fiber signal conditioner (Neoptix). The Neoptix T1 fiber optic temperature probe has a response time of less than 500 ms and the size of the sensitive area is 300 microns in diameter. Temperature recording was initiated once tissue temperature was stabilized prior to light-pulses.
Tajima K., Ikeda K., Tanabe Y., Thomson E.A., Yoneshiro T., Oguri Y., Ferro M.D., Poon A.S, & Kajimura S. (2020). Wireless optogenetics protects against obesity via stimulation of non-canonical fat thermogenesis. Nature Communications, 11, 1730.
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2

Measurement of Thermogenic Responses

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Mice under anesthesia with urethane (1.3 mg/kg) were positioned in a stereotaxic apparatus according to the mouse brain atlas (Franklin and Paxinos, 2008 ). A stainless steel cannula (outer diameter, 0.35 mm) and a 1 ml disposable syringe were connected with polyethylene tubing and were inserted perpendicularly into the right lateral ventricle (coordinates: 0.1 mm posterior to bregma, 0.1 mm lateral to the midline, and 1 mm ventral to the brain surface). Then, mice were left on a self-regulating heating pad to stabilize the rectal temperature (TRec) at 37°C and implanted with a type T thermocouple probe (IT-18, Physitemp, Clifton, NJ) in the rectum and with needle microprobes (MT-29/1, Physitemp) in the interscapular BAT and inguinal WAT (Yoneshiro et al., 2019 (link)). Tissue temperatures were simultaneously recorded by TC-2000 Meter (Sable Systems International). When tissue temperatures were stable, for >5 min, PGE2 (1.4 μg; Sigma) in 1.4 μl of pyrogen-free saline or only saline was injected into the ventricle through the cannula. The changes in tissue temperatures were contentiously measured every second for 1 hr or as indicated, and the mean value per minute was calculated.
Yoneshiro T., Kataoka N., Walejko J.M., Ikeda K., Brown Z., Yoneshiro M., Crown S.B., Osawa T., Sakai J., McGarrah R.W., White P.J., Nakamura K, & Kajimura S. (2021). Metabolic flexibility via mitochondrial BCAA carrier SLC25A44 is required for optimal fever. eLife, 10, e66865.
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3

Measuring Tissue Temperature in Mice

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Tissue temperature in the interscapular BAT and the skeletal muscle was recorded using the type T thermocouple probes and recorded by using the TC-2000 Meter (Sable Systems International), according to our protocol3 (link). Core body temperature was monitored using a TH-5 thermometer (Physitemp) by recording the rectal temperature of the mice. For core-body temperature recording experiment in Fig. 5i, both control (Ppargflox/flox) and PpargMyoD KO (Myod-CreERT2;Ppargflox/flox) mice were pre-treated with β-blocker and tamoxifen at room temperature for 5 days and acclimated to 15°C for additional 5 days. Wild-type mice did not receive pre-β-blocker-treatment. Subsequently, all the mice were acutely treated with β-blocker and exposed to 10°C.
Chen Y., Ikeda K., Yoneshiro T., Scaramozza A., Tajima K., Wang Q., Kim K., Shinoda K., Sponton C.H., Brown Z., Brack A, & Kajimura S. (2018). Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature, 565(7738), 180-185.
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4

Core and Tissue Temperature Monitoring in Transgenic Mice

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For core-body temperature recording experiments, rectal temperature of BckdhaUCP1 KO and Slc25a44 KD mice was monitored using a TH-5 thermometer (Physitemp) up to 14 hours after cold exposure. For tissue temperature recording, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, liver, and skeletal muscle, according to the method that was described previously 33 . Tissue temperature was recorded by TC-2000 Meter (Sable Systems International). When tissue temperature was stable, mice were intraperitoneally administered norepinephrine at a dose of 1 mg per kg body weight to induce non-shivering thermogenesis.
Yoneshiro T., Wang Q., Tajima K., Matsushita M., Maki H., Igarashi K., Dai Z., White P.J., McGarrah R.W., Ilkayeva O.R., Deleye Y., Oguri Y., Kuroda M., Ikeda K., Li H., Ueno A., Ohishi M., Ishikawa T., Kim K., Chen Y., Sponton C.H., Pradhan R.N., Majd H., Greiner V.J., Yoneshiro M., Brown Z., Chondronikola M., Takahashi H., Goto T., Kawada T., Sidossis L., Szoka F.C., McManus M.T., Saito M., Soga T, & Kajimura S. (2019). BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature, 572(7771), 614-619.
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5

Monitoring Tissue Temperatures in Mice

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The rectal temperature of mice was monitored by a TH-5 thermometer (Physitemp). For the recording of the tissue, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, and skeletal muscle, according to the previous study 12 . The temperature of each tissue was recorded by TC-2000 Meter (Sable Systems International). When the tissue temperature keeps stable, mice were treated with norepinephrine at a dose of 3 mg per kg body weight to induce non-shivering thermogenesis.
Tajima K., Ikeda K., Chang H.Y., Chang C.H., Yoneshiro T., Oguri Y., Jun H., Wu J., Ishihama Y, & Kajimura S. (2019). Mitochondrial lipoylation integrates age-associated decline in brown fat thermogenesis. Nature metabolism, 1(9), 886-898.
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6

Measuring Tissue Temperature in Mice

Cited 1 time
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Tissue temperature in the interscapular BAT and the skeletal muscle was recorded using the type T thermocouple probes and recorded by using the TC-2000 Meter (Sable Systems International), according to our protocol3 (link). Core body temperature was monitored using a TH-5 thermometer (Physitemp) by recording the rectal temperature of the mice. For core-body temperature recording experiment in Fig. 5i, both control (Ppargflox/flox) and PpargMyoD KO (Myod-CreERT2;Ppargflox/flox) mice were pre-treated with β-blocker and tamoxifen at room temperature for 5 days and acclimated to 15°C for additional 5 days. Wild-type mice did not receive pre-β-blocker-treatment. Subsequently, all the mice were acutely treated with β-blocker and exposed to 10°C.
Chen Y., Ikeda K., Yoneshiro T., Scaramozza A., Tajima K., Wang Q., Kim K., Shinoda K., Sponton C.H., Brown Z., Brack A, & Kajimura S. (2018). Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature, 565(7738), 180-185.
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7

Monitoring Tissue Temperatures in Mice

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The rectal temperature of mice was monitored by a TH-5 thermometer (Physitemp). For the recording of the tissue, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, and skeletal muscle, according to the previous study 12 . The temperature of each tissue was recorded by TC-2000 Meter (Sable Systems International). When the tissue temperature keeps stable, mice were treated with norepinephrine at a dose of 3 mg per kg body weight to induce non-shivering thermogenesis.
Tajima K., Ikeda K., Chang H.Y., Chang C.H., Yoneshiro T., Oguri Y., Jun H., Wu J., Ishihama Y, & Kajimura S. (2019). Mitochondrial lipoylation integrates age-associated decline in brown fat thermogenesis. Nature metabolism, 1(9), 886-898.
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8

Core and Tissue Temperature Monitoring in Transgenic Mice

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For core-body temperature recording experiments, rectal temperature of BckdhaUCP1 KO and Slc25a44 KD mice was monitored using a TH-5 thermometer (Physitemp) up to 14 hours after cold exposure. For tissue temperature recording, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, liver, and skeletal muscle, according to the method that was described previously 33 . Tissue temperature was recorded by TC-2000 Meter (Sable Systems International). When tissue temperature was stable, mice were intraperitoneally administered norepinephrine at a dose of 1 mg per kg body weight to induce non-shivering thermogenesis.
Yoneshiro T., Wang Q., Tajima K., Matsushita M., Maki H., Igarashi K., Dai Z., White P.J., McGarrah R.W., Ilkayeva O.R., Deleye Y., Oguri Y., Kuroda M., Ikeda K., Li H., Ueno A., Ohishi M., Ishikawa T., Kim K., Chen Y., Sponton C.H., Pradhan R.N., Majd H., Greiner V.J., Yoneshiro M., Brown Z., Chondronikola M., Takahashi H., Goto T., Kawada T., Sidossis L., Szoka F.C., McManus M.T., Saito M., Soga T, & Kajimura S. (2019). BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature, 572(7771), 614-619.
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9

Measuring Tissue Temperatures in Mice

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Rectal temperature of the mice was monitored using a TH-5 thermometer (Physitemp). For tissue temperature recording, mice under anesthesia were implanted with the type T thermocouple probes at interscapular BAT, inguinal WAT, and skeletal muscle. Temperature in skeletal muscle on the back was record according to the previous study 51 . The temperature at each tissue was recorded by TC-2000 Meter (Sable Systems International). Core body temperature was maintained at 37°C or above. When tissue temperature reached stable, animals were treated with norepinephrine at a dose of 1 mg/kg.
Ikeda K., Kang Q., Yoneshiro T., Camporez J.P., Maki H., Homma M., Shinoda K., Chen Y., Lu X., Maretich P., Tajima K., Ajuwon K.M., Soga T, & Kajimura S. (2017). UCP1-independent signaling involving SERCA2b-mediated calcium cycling regulates beige fat thermogenesis and systemic glucose homeostasis. Nature medicine, 23(12), 1454-1465.
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10

Measuring Tissue Temperatures in Mice

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Rectal temperature of the mice was monitored using a TH-5 thermometer (Physitemp). For tissue temperature recording, mice under anesthesia were implanted with the type T thermocouple probes at interscapular BAT, inguinal WAT, and skeletal muscle. Temperature in skeletal muscle on the back was record according to the previous study 51 . The temperature at each tissue was recorded by TC-2000 Meter (Sable Systems International). Core body temperature was maintained at 37°C or above. When tissue temperature reached stable, animals were treated with norepinephrine at a dose of 1 mg/kg.
Ikeda K., Kang Q., Yoneshiro T., Camporez J.P., Maki H., Homma M., Shinoda K., Chen Y., Lu X., Maretich P., Tajima K., Ajuwon K.M., Soga T, & Kajimura S. (2017). UCP1-independent signaling involving SERCA2b-mediated calcium cycling regulates beige fat thermogenesis and systemic glucose homeostasis. Nature medicine, 23(12), 1454-1465.
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