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Rabbit polyclonal anti erk1 2

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Rabbit polyclonal anti-ERK1/2 is an antibody that recognizes the ERK1 and ERK2 proteins. ERK1/2 are serine/threonine protein kinases that play a key role in the MAPK/ERK signaling pathway.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg horseradish peroxidase, by Santa Cruz Biotechnology (2 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Lipopolysaccharide lps rough strains from escherichia coli f583 rd mutant, by Merck Group (2 mentions) Mouse monoclonal anti phospho erk1 2, by Cell Signaling Technology (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Rabbit monoclonal gsk3β, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti gapdh, by Merck Group (1 mentions) Rabbit monoclonal anti akt, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti mek1 2, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti flag, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) Sp600125, by Merck Group (1 mentions) U0126, by Merck Group (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Rhodamine conjugated phalloidin, by Cytoskeleton (1 mentions) Anti ho 1, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

ERK1/2 (1487 citations) Anti-ERK1/2 (692 citations) Rabbit anti-ERK1/2 (92 citations) Rabbit polyclonal anti-phospho-ERK1/2 (5 citations) Polyclonal anti-ERK1/2 (4 citations) Rabbit polyclonal anti-total ERK1/2 (3 citations) Rabbit anti-ERK1/2 polyclonal antibody (2 citations)

6 protocols using rabbit polyclonal anti erk1 2

1

Antibody-Based Western Blotting Methodology

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Western blotting was performed as previously reported [61 (link), 62 (link)]. The antibodies used were rabbit polyclonal anti-phospho-Ser473-Akt (#9271, 1:1000, Cell Signaling Technology, Danvers MA, USA), rabbit monoclonal anti-Akt (#4691, 1:1000, Cell Signaling Technology), rabbit polyclonal phospho-Ser9-GSK3β (#9336, 1:1000, Cell Signaling Technology), rabbit monoclonal GSK3β (#9315, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-phospho-Thr202/Tyr204-ERK1/2 (#9101, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-ERK1/2 (#9102, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-phospho-Ser217/221-MEK1/2 (#9121, Cell Signaling Technology), rabbit polyclonal anti-MEK1/2 (#9122, Cell Signaling Technology), rabbit polyclonal anti-p16 (10883-1-AP, 1:500, Proteintech, Rosemont, IL, USA), rat monoclonal anti-p19 (sc-32748, 1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-p21 (ab7960, Abcam, Cambridge, UK), goat polyclonal anti-IGFBP5 (sc-6006, 1:200, Santa Cruz Biotechnology), mouse monoclonal anti-FLAG (F3165, 1:1000, Sigma Aldrich; Merck KGaA, Darmstadt, Germany), mouse monoclonal anti-β-actin (010-27841, 1:10000, Fujifilm Wako Pure Chemical Corporation, Osaka, Japan), and mouse monoclonal anti-GAPDH (G8795, 1:10000, Sigma Aldrich, St. Louis, MO, USA).
Nojima I., Hosoda R., Toda Y., Saito Y., Ueda N., Horimoto K., Iwahara N., Horio Y, & Kuno A. (2022). Downregulation of IGFBP5 contributes to replicative senescence via ERK2 activation in mouse embryonic fibroblasts. Aging (Albany NY), 14(7), 2966-2988.
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2

Antibody Validation for Epithelial-Mesenchymal Transition

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U0126, SP600125, and SB203580 were purchased from Millipore (Billerica, MA, USA). Rabbit polyclonal anti‐ZEB1 and anti‐ZEB2 antibodies were from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti–Ets1 (C20) was from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit polyclonal anti‐phospho‐Ets1 (phospho‐T38) antibodies and monoclonal anti‐ESE1 antibody were from Abcam (Cambridge, UK). Rabbit polyclonal anti‐ERK1/2 and anti‐phospho‐ERK1/2 antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal anti‐E‐cadherin and anti‐N‐cadherin antibodies were from BD Transduction Laboratories (Lexington, KY, USA). Mouse monoclonal anti‐α‐tubulin and anti‐Flag (M2) antibodies were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Rhodamine‐conjugated phalloidin was purchased from Cytoskeleton (Denver, CO, USA).
Sinh N.D., Endo K., Miyazawa K, & Saitoh M. (2017). Ets1 and ESE1 reciprocally regulate expression of ZEB1/ZEB2, dependent on ERK1/2 activity, in breast cancer cells. Cancer Science, 108(5), 952-960.
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3

Antioxidant Pathways Regulation by LPS

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Lipopolysaccharide (LPS) (rough strains) from Escherichia coli F583 (Rd mutant) and fetal bovine serum (FBS) were obtained from Sigma-Aldrich (St. Louis, MO). FBS (lot no. 12J002) was qualified USA origin, sterile, filtered and cell culture tested, and certified to contain endotoxin levels less than 1.0 ng/ml. Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, 0.05% (w/v) trypsin/EDTA, and phosphate-buffered saline (PBS) were obtained from GIBCO (Gaithersburg, MD). Tin Protoporphyrin IX dichloride (TinPPIX) was from Santa Cruz Biotechnology (Santa Cruz, CA). Kinase inhibitors (U0126 and SB202190) were from Cell Signaling (Beverly, MA).
Antibodies used for Western blots include: goat anti-rabbit IgG-horseradish peroxidase, goat anti-mouse IgG-horseradish peroxidase, rabbit polyclonal anti-Nrf2, and anti-HO-1 (Santa Cruz Biotechnology, Santa Cruz, CA); mouse monoclonal anti-β-actin (Sigma-Aldrich, St. Louis, MO); rabbit polyclonal anti-ERK1/2, mouse monoclonal anti-phospho-ERK1/2, rabbit monoclonal anti-p38MAPK, and anti-phospho-p38MAPK (Cell Signaling, Beverly, MA). Quercetin (Sigma-Aldrich, St. Louis, MO) and cyanidin chloride (Indofine Chemical Comp., Hillsborough, NJ) were dissolved in DMSO as a stock solution.
Sun G.Y., Chen Z., Jasmer K.J., Chuang D.Y., Gu Z., Hannink M, & Simonyi A. (2015). Quercetin Attenuates Inflammatory Responses in BV-2 Microglial Cells: Role of MAPKs on the Nrf2 Pathway and Induction of Heme Oxygenase-1. PLoS ONE, 10(10), e0141509.
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4

Macrophage Inflammatory Response Assay

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Dulbecco’s modified Eagle’s medium (DMEM), penicillin, streptomycin, 0.05% (w/v) trypsin/EDTA, and phosphate-buffered saline (PBS) were obtained from GIBCO (Gaithersburg, MD). Interferon-γ (IFNγ) was purchased from R & D Systems (Minneapolis, MN). Lipopolysaccharide (LPS) (rough strains) from Escherichia coli F583 (Rd mutant) and methylthiazolyldiphenyl-tetrazolium bromide (MTT) were from Sigma-Aldrich (St. Louis, MO). WST-1 kit for assay of cell viability was obtained from Clontech (Mountain View, CA). Fetal bovine serum was from Atlanta Biologicals (Lawrenceville, GA). Antibodies used for Western blots include: goat anti-rabbit IgG- horseradish peroxidase, goat anti-mouse IgG- horseradish peroxidase and anti-iNOS rabbit polyclonal (Santa Cruz Biotechnology, Santa Cruz, CA); monoclonal anti-β-actin peroxidase (Sigma-Aldrich, St. Louis, MO); STAT1α rabbit polyclonal antibody (Millipore, Billerica, MA), rabbit polyclonal p-STAT1 pSer727 (Pierce Biotechnology, Rockford, IL), rabbit polyclonal anti-ERK1/2, and mouse monoclonal anti-phospho-ERK1/2, (Cell Signaling, Beverly, MA). For ROS detection, CM-H2DCF-DA (DCF) was obtained from Invitrogen, Inc. (Eugene, OR), and dihydroethidium (DHE) from Sigma-Aldrich (St. Louis, MO).
Jiang J., Chuang D.Y., Zong Y., Patel J., Brownstein K., Lei W., Lu C.H., Simonyi A., Gu Z., Cui J., Rottinghaus G.E., Fritsche K.L., Lubahn D.B., Folk W.R, & Sun G.Y. (2014). Sutherlandia frutescens Ethanol Extracts Inhibit Oxidative Stress and Inflammatory Responses in Neurons and Microglial Cells. PLoS ONE, 9(2), e89748.
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5

Protein Extract Isolation and Analysis

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Protein extracts were isolated using TRIzol Reagent (Invitrogen) following manufacturer's indications, clarified (12,000xg, 15min, 4°C), denatured and subjected to SDS-PAGE and Western-blot. Antibodies: rabbit polyclonal anti-SET (Abcam), rabbit monoclonal anti-PP2AY307 (Epitomics), rabbit polyclonal anti-AKT, rabbit polyclonal anti-ERK1/2 (Cell Signaling Technology), rabbit polyclonal anti-pAKT, rabbit poly-clonal anti-pERK1/2 (Santa Cruz Biotechnology), rabbit polyclonal anti-CIP2A, and mouse monoclonal anti-β-Actin (Sigma). Proteins were detected with the appropriate secondary antibodies conjugated to alkaline phospatase (Sigma) by chemiluminescence using Tropix CSPD and Tropix Nitro Block II (Applied Biosystems).
Rincón R., Cristóbal I., Zazo S., Arpí O., Menéndez S., Manso R., Lluch A., Eroles P., Rovira A., Albanell J., García-Foncillas J., Madoz-Gúrpide J, & Rojo F. (2015). PP2A inhibition determines poor outcome and doxorubicin resistance in early breast cancer and its activation shows promising therapeutic effects. Oncotarget, 6(6), 4299-4314.
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6

Antibodies for Stem Cell Signaling

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Rabbit polyclonal anti-Nanog, rabbit monoclonal anti-ACLY (EP704Y), and rabbit monoclonal phospho-ACLY (EP737Y) antibodies were purchased from Abcam (Cambridge, UK). Mouse monoclonal anti-Stat3 (124H6), rabbit monoclonal anti-phospho-Stat3 (Y705) (D3A7), rabbit polyclonal anti-ERK1/2, and rabbit polyclonal anti-phospho-ERK1/2 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-β-actin (AC-74) antibody was obtained from Sigma. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare UK (Little Chalfont, UK).
Kobayashi H., Nishimura H., Kudo N., Osada H, & Yoshida M. (2020). A novel GSK3 inhibitor that promotes self-renewal in mouse embryonic stem cells. Bioscience, biotechnology, and biochemistry, 84(10).
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