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Tsq quantum ultra triple

Manufactured by Thermo Fisher Scientific
Sourced in United States

The TSQ Quantum Ultra is a triple quadrupole mass spectrometer designed for high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC) applications. It provides accurate and sensitive detection of target compounds in complex matrices, enabling precise quantitative analysis.

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4 protocols using tsq quantum ultra triple

1

Lipid Quantification by HPLC-MS/MS

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Lipids were extracted from 50 μl of liver homogenate with an internal standard mixture. The extracted free fatty acids were derivatized by amino methyl phenyl pyridium (AMPP) into FA-AMPP derivatives in order to obtain high sensitivity in MS. Measurement of lipids was performed with a Shimadzu 10A HPLC system and a Shimadzu SIL-20AC HT auto-sampler coupled to a Thermo Scientific TSQ Quantum Ultra triple quadrupole mass spectrometer operated in SRM mode under ESI(+). Data processing was conducted with Xcalibur (Thermo).
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2

25OHD3 Quantification by HPLC-MS

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The vitamin D metabolite 25-hydroxy D3 (25OHD3) was measured by high performance liquid chromatography mass spectrometry. The high performance liquid chromatography system was a Transcend TLX turboflow 2 system attached to a TSQ Quantum Ultra triple quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).
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3

Sphingolipid Profiling by HPLC-MS/MS

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Sphingolipid mass spectrometry analysis was carried out as previously reported (90 (link)). Briefly, a Thermo Accela high-performance liquid chromatography (HPLC) system (San Jose, CA) was used to separate the dried extracts dissolved in 150 μL of ammonium formate (1 mM) with 0.2% of formic acid in methanol. A Peeke Scientific Spectra C8 (Redwood City, CA) HPLC column (150 × 3 mm) was used, into which 10 μL of samples were injected. The HPLC was coupled to the HESI source of a Thermo TSQ Quantum Ultra triple quadrupole mass spectrometer (San Jose, CA). The sphingolipid profile was performed using positive ion mode, with the high voltage set to 3.5 kV, vaporizer temperature at 400°C, sheath gas pressure at 60, auxiliary gas pressure at 15, and a capillary temperature of 300°C. The collision cell was operated at 1.5 mTorr of argon. For the duration of the run, transitions for each lipid species were monitored at 100- or 50-ms dwell time. A total of 20 lipid standards for our profile from Avanti (Alabaster, AL) were used to develop calibration curves, and these curves were then used for lipid species to be monitored. Processing of the samples was done using Thermo Xcalibur 2.2 Quan browser software and exported to Excel for reporting results. Sphingolipid concentration determined by mass spectrometry was further normalized by the Pi abundance (91 (link)).
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4

LC-MS/MS Quantification of Ponatinib

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The LC-MS/MS equipment consisted of an Accela pump and autoinjector and a TSQ Quantum Ultra triple quadrupole mass spectrometer with heated electrospray ionization (HESI), all supplied by Thermo Fisher Scientific, San Jose, CA, USA. Data were recorded and the system was controlled using the Thermo Fisher Xcalibur software (version 2.07). Fig. 2. SRM chromatograms of ponatinib, metabolite and both labeled internal standards at the LLOQ. For each transition the response of the corresponding double blank sample has been added. An artificial off-set was given to all traces.
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