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4 protocols using 3 aminopropyl triethoxysilane aps

1

Synthesis of γ-Fe2O3/SiO2/RhB Nanoparticles

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For the synthesis of the γ-Fe2O3/SiO2/RhB NPs, reagent grade chemicals were used as received from the manufacturers. Iron (III) sulfate hydrate, iron (II) sulfate heptahydrate (ACS, 99%), citric acid (CA, 99%), tetraethoxysilane (TEOS, 99.9%) and NH4OH (28–30%) were supplied by Alfa Aesar (Lancashire, UK). Acetone (AppliChem GmbH) and absolute ethanol (Carlo Erba, reagent - USP) were used as received. (3-Aminopropyl)triethoxysilane (APS; silane-NH2, 99%), tetraethoxysilane (TEOS; 98%), ethyl acetate (EA), dichloromethane (DCM), dimethylformamide (DMF), Rhodamine B isothiocyanate (RhB), poly(acrylic acid) solution (25 wt. % in water) and polyvinyl pyrrolidone (PVP, 40 kDa) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Cellulose acetate (CA, Mn = 30000 g/mol) was obtained from Sigma-Aldrich and used without further purification. Acetone (Technical Grade 99.5% - Panaska Trading CO) was the solvent used in the preparation of CA polymer solutions that were further electrospun.
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2

Labeling Algae Cells for Flow Cytometry

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Water was obtained using a Millipore apparatus. Other reagents were rhodamine B (RhB) and fluorescein (Fl) (99% purity, Sigma-Aldrich), tetraethyl orthosilicate (TEOS) (98%, Sigma-Aldrich), ethanol (Sintorgan, HPLC grade), 3-(aminopropyl)triethoxysilane (APS) (98%, Sigma-Aldrich), N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (98%, Sigma-Aldrich) and sodium cyanide (95%, Sigma-Aldrich).
Ultra-filtrated and deionized water was obtained using a Millipore apparatus.
Micrometer multicolor beads from BD Company were used as control particles for in-flow cytometry.
The wild population of Microcystis aeruginosa was concentrated from an atoxic bloom collected in San Roque water reservoir (Cordoba, Argentina).
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3

Synthesis of Cobalt-based Nanoparticles

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Cobalt chloride hexahydrate (CoCl2 · 6H2O), sodium borohydride (NaBH4), sodium citrate dehydrate, and anhydrous ethanol were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd., China. TEOS and 3-aminopropyltriethoxysilane (APS) were purchased from Sigma-Aldrich, St. Louis, MO, USA. All reagents were used as received. Deionized water was distilled by a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA).
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4

Bioconjugation of TRC105 with IR Dye 800CW

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TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). IR Dye 800CW-NHS (NHS denotes N-hydroxysuccinimide) ester was acquired from LI-COR Biosciences Co. (Lincoln, NE). SCM-PEG5k-Mal was obtained from Creative PEGworks. NOTA-SCN (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), ammonia (NH3·H2O), triethylamine (TEA), 3-aminopropyl)triethoxysilane (APS), dimethyl sulfoxide, cetyltrimethylammonium chloride (CTAC, 25 wt %), and Kaiser test kit were purchased from Sigma-Aldrich (St. Louis, MO). Traut’s reagent (2-Iminothiolane·HCl) and Ellman’s reagent (5,5′-dithiobis(2-nitrobenzoic acid) or DTNB) were purchased from Fisher Scientific. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
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