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Rat mouse gh elisa

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The Rat/Mouse Growth Hormone (GH) ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of growth hormone levels in rat and mouse serum or plasma samples. The assay employs specific antibodies and a colorimetric detection system to determine the concentration of GH in the tested samples.

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Rat/mouse GH ELISA kit ELISAs

2 protocols using rat mouse gh elisa

1

Quantifying Rat GH in GH3 Cells

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GH3 cells were plated in duplicates in six-well plates at a density of 1.5 × 105 cells/well. The following day and after 24 h from the first treatment, cell culture medium was replaced with medium containing OCT and PAS or vehicle as CTR. After 24 and 48 h from the first treatment, cell culture media were collected and stored at − 80° C until analyzed. Rat GH was measured by a rat/mouse GH ELISA (EMD Millipore, Billerica, Massachusetts, cat. #EZRMGH-45K) according to the manufacturer’s procedure.
Dicitore A., Saronni D., Gaudenzi G., Carra S., Cantone M.C., Borghi M.O., Persani L, & Vitale G. (2021). Long-term effects of somatostatin analogues in rat GH-secreting pituitary tumor cell lines. Journal of Endocrinological Investigation, 45(1), 29-41.
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2

Murine Growth Hormone ELISA Quantification

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Murine GH was measured by a rat/mouse GH ELISA (EMD Millipore, Billerica,
Massachusetts, USA) in medium collected from the GC cultures, post-transfected
with siRNAs according to the manufacture’s instructions. GH levels
were analyzed in quadruplicate.
Bouzo-Lorenzo M., Santo-Zas I., Lodeiro M., Nogueiras R., Casanueva F.F., Castro M., Pazos Y., Tobin A.B., Butcher A.J, & Camiña J.P. (2016). Distinct phosphorylation sites on the ghrelin receptor, GHSR1a, establish a code that determines the functions of ß-arrestins. Scientific Reports, 6, 22495.
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