Her 2 protein

Anti-EGFR, anti-phospho-EGFR, anti-phospho-HER2, anti-HER2, anti-phopho-akt, anti-akt monoclonal antibodies were purchased from Cell Signaling Technology. Anti-GAPDH monoclonal antibody (sc-365052) was purchased from Santacruz Biotechnology (Dallas, TX, USA). Cell-titer Glo assay kit was purchased from Promega (Madision, MI, USA). HER2 protein and EGFR protein for surface plasmon resonance study was obtained from Abcam and Leinco Technologies Inc, respectively. 1× RBC lysis buffer (420301, Biolegend, San Diego, CA); Concanavalin A (ConA; C5275, Sigma-Aldrich, Saint Louis, MO, USA); viability dye (LIVE/DEAD^™ Fixable Near-IR Dead Cell Stain Kit, L34975, Invitrogen, Eugene, OR, USA); Fixation/Permeabilization solution (00-5123-43 and 00-5223-56, eBioscience, Carlsbad, CA, USA); CD3-PerCP-eFlour710 (17A2, Invitrogen); CD4-FITC (GK 1.5, BD Biosciences); CD8a-BV650 (53–6.7, BD Horizon); and Ki67-AF647(B56, BD Pharmingen) for peptide immunogenicity study were purchased. Proximity ligation assay was performed using the Duolink® II assay kit (Sigma Aldrich). Primary antibodies for EGFR, HER2 and HER3 were from Enzo Life Sciences.