Glr001

Manufactured by R&D Systems

Sourced in United States

The GLR001 is a general-purpose laboratory instrument designed for liquid handling and pipetting applications. It provides precise and accurate liquid transfers with adjustable volume ranges. The GLR001 is suitable for use in a variety of laboratory settings, including research, clinical, and diagnostic environments.

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Lab products found in correlation

Ab50011, by Abcam (1 mentions) Ab17942, by Abcam (1 mentions) Ab189524, by Abcam (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Hyaluronic acid, by R&D Systems (1 mentions) P yap, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) Flag m2, by Merck Group (1 mentions)

3 protocols using glr001

CD44-Mediated Signaling Activation

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SK-Hep1 cells were incubated with either hyaluronan (HA) or peptide to evaluate activation of downstream signaling after binding to CD44. 100 μg/mL of low molecular weight HA (GLR001, R&D Systems) was added for 15 min. Peptides are added at concentrations of 4 and 300 μM for 15 min. Anti-CD44 antibody (#ab189524, Abcam), anti-AKT (#4691, Cell Signaling), anti-phospho-AKT (#9271, Cell Signaling), anti-ERK1/2 (#ab17942, Abcam,), anti-phospho-ERK1/2 (#ab50011, Abcam), and anti-β-Actin (#4967, Cell Signaling Technology) were used per manufacturer’s instructions.

Wu X., Meng X., Chang T.S., Feng S., Lee M., Jaiswal S., Choi E.Y., Tran L., Jiang H, & Wang T.D. (2022). Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma. Photoacoustics, 26, 100355.

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Autophagic Flux Modulation by HA Treatment

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Cells were plated at a density of 4 million cells per well on 6 well plates. Cells were then differentiated with RA for 7 days. On the day prior to lysis, cells were treated with high (cat# GLR004, R&D Systems) or low molecular weight (cat# GLR001, R&D Systems) HA in serum-free media and then lysed using RIPA lysis buffer, as described above. For autophagic flux assays, cells were treated with or without HA in the presence or absence of Bafilomycin A1 (100nM) for 4 hours immediately prior to lysis and then autophagic flux was assessed as described above.

Rahman A.A., Soto-Avellaneda A., Jin H.Y., Stojkovska I., Lai N.K., Albright J.E., Webb A.R., Oe E., Valarde J.P., Oxford A.E., Urquhart P.E., Wagner B., Brown C., Amado I., Vasquez P., Lehning N., Grozdanov V., Pu X., Danzer K.M, & Morrison B.E. (2020). Enhanced hyaluronan signaling and autophagy dysfunction by VPS35 D620N. Neuroscience, 441, 33-45.

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Hyaluronic Acid Regulation of YAP/TAZ Signaling

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Fluvastatin sodium (PHR1620) was obtained from Sigma Aldrich (St Louis, MO, USA). Hyaluronic acid (Molecular Weight: 15-40 kDa) (GLR001) was obtained from R&D Systems (Minneapolis, MN, USA). Primary antibodies against YAP (#14074, 1:100 for immunofluorescence or 1:1000 for Western blotting; Cell Signaling Technology, Danvers, MA, USA), p-YAP (#13008, 1:1000 for Western blotting; Cell Signaling Technology), TAZ (560235, 1:500 for Western blotting; BD Pharmingen, Franklin Lakes, NJ, USA), RHAMM (CD168) (ab170527, 1:250 for Western blotting; Abcam, Cambridge,UK), α-tubulin (T5168, 1:4000 for Western blotting; Sigma Aldrich), and FLAG M2 (F3165, 1:1000 for Western blotting; Sigma Aldrich), and DAPI solution (D523, 1:1000 for nuclear staining, Dojindo Laboratories, Kumamoto, Japan) were used in accordance with the manufacturer’s instructions. Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey (NA934), and Anti-Mouse IgG, HRP-Linked Whole Ab Sheep (NA931) were obtained from GE Healthcare (Little Chalfont, Buckinghamshire, UK).

Shigeeda W., Shibazaki M., Yasuhira S., Masuda T., Tanita T., Kaneko Y., Sato T., Sekido Y, & Maesawa C. (2017). Hyaluronic acid enhances cell migration and invasion via the YAP1/TAZ-RHAMM axis in malignant pleural mesothelioma. Oncotarget, 8(55), 93729-93740.

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