Ethyl acetate, n-hexane, tetrahydrofuran (THF), diethyl ether, N,N‐dimethylformamide (DMF, anhydrous), l -alanine, l -isoleucine, bis(trichloromethyl) carbonate, glycine, trichloromethyl chloroformate, Proteinase K, Trypsin and naproxen (Npx) were purchased from Sigma-Aldrich (USA). Monomethoxy-monoamino-terminated poly(ethylene glycol) (mPEG45-NH2, Mn = 2000 g/mol) was purchased from Rapp Polymere (Germany). All chemicals were used without further purification, unless otherwise noted. Nanopure water (18.2 MΩ•cm) was acquired from a Milli-Q water filtration system, Millipore Corp. (St. Charles, MO). Tris-HCl buffered saline and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (USA) as dry powders, and dissolved in deionized water (1.0 L) to obtain the desired buffer solutions.
Tris hcl buffered saline
Manufactured by Merck Group
Sourced in Switzerland
Tris-HCl buffered saline is a common laboratory buffer solution used to maintain a stable pH environment for various biological applications. It consists of a mixture of Tris (tris(hydroxymethyl)aminomethane) and hydrochloric acid (HCl), which together form a buffering system capable of maintaining a near-neutral pH. This buffer solution is widely used in a variety of biochemical and molecular biology techniques, such as protein purification, enzyme assays, and cell culture media preparation.
Automatically generated - may contain errors
Lab products found in correlation
A030 2, by Nanjing Jiancheng (2 mentions)
A003 1, by Nanjing Jiancheng (2 mentions)
Naproxen npx, by Merck Group (1 mentions)
Milli q water filtration system, by Merck Group (1 mentions)
Nanopure water, by Merck Group (1 mentions)
Bis trichloromethyl carbonate, by Merck Group (1 mentions)
L isoleucine, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Proteinase k, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Au680, by Beckman Coulter (1 mentions)
4 protocols using tris hcl buffered saline
1
Peptide Conjugation Using PEG Linker
2
Liver Biomarker Quantification Protocol
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The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), total bilirubin (TBIL), alkaline phosphatase (ALP), and gamma glutamyl transpeptidase (γ-GT) were assessed using the Automated Biochemical Analyzer (AU-680, Beckman, Germany). Liver homogenate (10%, w/v) was prepared by homogenizing the right lobe of liver on ice in 150 mM Tris-HCl buffered saline (pH 7.2; Sigma-Aldrich) using a polytron homogenizer (PT3100D; Kinematical, Lucerne, Switzerland). The levels of hydroxyproline (Hyp) and malondialdehyde (MDA) in liver tissue were measured using kits (NanJing JianCheng Bioengineering Institute, A030-2, A003-1, Nanjing, China) according to the manufacturer’s instructions.
3
Measuring Antioxidant Enzyme Activity and Lipid Peroxidation in Liver Tissues
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Liver homogenate (10%, w/v) was prepared by homogenizing the liver tissue on ice in 150 mM Tris-HCl buffered saline (pH 7.2; Sigma-Aldrich) using a polytron homogenizer (PT3100D; Kinematical, Lucerne, Switzerland). A commercially available kit (Jiancheng Biological Engineering Research Institute, Nanjing, China) was used to determine the activities of SOD and GSH-Px according to the manufacturer’s instructions. Data are expressed as SOD U/mg protein and GSH-Px mg/g protein (26 (link)). MDA levels in liver tissues were determined using the thiobarbituric acid method, provided by a commercially available kit (Jiancheng Biological Engineering Research Institute) and measured according to the manufacturer’s instructions.
4
Liver Injury Biomarkers Evaluation
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Rat blood samples were taken at 4 weeks after cell transplantation and the serum was collected. Then, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltranspeptidase (γ-GT), direct bilirubin (DBIL), total bilirubin (TBIL), total protein (TP) and albumin (ALB) concentrations were assessed using an automated biochemical analyzer (AU-680, Beckman, Germany). Liver homogenate (10%, w/v) was prepared by homogenizing the right lobe of the liver on ice in 150 mM Tris-HCl buffered saline (pH 7.2; Sigma-Aldrich) using a polytron homogenizer (PT3100D; Kinematical, Lucerne, Switzerland). Next, the levels of hydroxyproline (HYP) and malondialdehyde (MDA) were measured using kits (NanJing JianCheng Bioengineering Institute, A030-2, A003-1, Nanjing, China) according to the manufacturer's instructions.
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