Anti tg2 antibody

All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl₂ and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).

Lung tissues were fixed in 4% paraformaldehyde, processed, and embedded in paraffin. We carefully examined the cores and inserted them into new paraffin blocks, using Tissue Arrayer Minicore (Alphelus, Plaisir, France). We deparaffinized the sections with a thickness of 5 μm and washed them with 100% ethanol, 90% ethanol, 70% ethanol, and then distilled water. These sections were then prepared for antigen retrieval in a citrate buffer with a pH of 6.0 by heating them in a microwave for 5-min cycles. The sections were then incubated overnight at a temperature of 4°C with a 1:200 diluted Anti-TG2 antibody (Abcam, USA), after which we incubated them with biotin-labeled Rabbit Anti-Mouse IgG H&L preabsorbed (Abcam, USA) for immunostaining (Chihong, et al., 2017 (link)).

All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl₂ and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).