IgG antibodies against Diphtheria Toxin were measured using Novagnost® Diphtheria Toxin 5S IgG (NovaTec Immunodiagnostica GmbH, Dietzenbach, Germany). IgG antibodies against Tetanus Toxoid were measured using Anti-Tetanus Toxoid ELISA (IgG) (EUROIMMUN, Lübeck, Germany), and IgG antibodies against Bordetella pertussis toxin were measured using Anti-Bordetella pertussis toxin ELISA (IgG) (EUROIMMUN). The samples were analyzed in the HUS Diagnostic Center Clinical Microbiology, Helsinki, Finland, with a fully automatic EuroLabWorkstation ELISA (EUROIMMUN), and the analysis was carried out according to the manufacturer's instructions.
Eurolabworkstation elisa
Manufactured by EUROIMMUN
Sourced in Finland, Germany
The EuroLabWorkstation ELISA is a fully automated ELISA processing system designed for high-throughput analysis. It automates the key steps of the ELISA process, including sample and reagent pipetting, incubation, washing, and absorbance measurement. The system is capable of processing multiple ELISA plates simultaneously, improving efficiency and productivity in the laboratory.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using eurolabworkstation elisa
1
Serological Assessment of Vaccine Antibodies
2
SARS-CoV-2 IgG Antibody Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood specimens were centrifuged for 5 min and 4,000×g. Serum was collected for analysis. Determination of SARS-CoV-2 specific IgG antibodies was performed using the Anti-SARS-CoV-2 ELISA (IgG) (EI 2606–9601 G, EUROIMMUN) and the Anti-SARS-CoV-2 NCP-ELISA (EI 2606-9601-2 G, EUROIMMUN) performed on the EUROLabWorkstation ELISA (EUROIMMUN) according to the manufacturer's instructions, which include a 1:101 dilution step of the serum. Analysis of paired serum, DBS_D and DBS_ND samples were performed on the same day.
3
SARS-CoV-2 Antibody Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We assessed anti-SARS-CoV-2 IgG antibodies using a commercially available ELISA (Euroimmun; Lübeck, Germany #EI 2606-9601 G) targeting the S1 domain of the spike protein of SARS-CoV-2; sera diluted 1:101 were processed on a EuroLabWorkstation ELISA (Euroimmun). An in-house validation study, using a set of sera from 176 pre-pandemic negative controls and 181 RT-PCR-confirmed COVID-19 cases was conducted to estimate test performance.10 (link) This validation study found that the manufacturer's recommended cutoff for positivity (>1·1) had a sensitivity of 93% and a specificity of 100%. As a confirmatory test, we used a recombinant immunofluorescence assay for all potentially indeterminate individuals (those with a ratio of optical density of clinical sample to optical density of internal calibrator between 0·5 and 1·5)10 (link) and all ELISA positives. We used only the ELISA results for estimating seroprevalence in our primary analyses but relied on the combined recombinant immunofluorescence and ELISA algorithm in sensitivity analyses (appendix pp 1, 7 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!