Ana-1 cells were grown in 25 cm2 flasks at a density of 3×106 cells/flask and incubated for 4 hours with FI-labeled PEGylated Au DENPs with 100 and 300 μM Au at 37°C. The control flasks were treated with RPMI 1640 medium alone. After washing, the cells were trypsinized and resuspended in a 0.5 mL Eppendorf tube containing approximately 5.0×106 Ana-1 cells. The cell pellets in each tube were scanned using a micro-CT (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA) according to the manufacturer instructions.
Micro ct
Manufactured by GE Healthcare
Sourced in United States, Canada
Micro-CT is a high-resolution imaging technology that uses X-rays to produce detailed three-dimensional images of small samples. It allows for the non-destructive visualization and analysis of the internal structure and composition of a wide range of materials, from biological specimens to engineered components.
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Lab products found in correlation
21 protocols using micro ct
1
Quantifying Cellular Uptake of PEGylated Au DENPs
2
Microstructural Analysis of ECM-Derived Scaffolds
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To observe the microstructure of the ECM-derived DCM/DCBM biphasic scaffold, specimens were cut from the scaffolds and the interior microstructure of cross-sections was investigated by scanning electron microscopy (SEM, Hitachi S-520, Japan) after coating with gold-palladium. Porosity was measured by the ethanol intrusion method. To observe the inner microstructure of the scaffold, we used micro-CT (GE Medical Systems, London, ON, Canada) [11 (link)]. Specimens were immersed in a 3% OsO4 solution to increase the X-ray attenuation and then air-dried.
3
Microcomputed Tomography Analysis of Heterotopic Ossification
4
Micro-CT Femur Bone Analysis
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The left posterior femur was scanned by Micro-CT (GE Healthcare company, boston, MA, USA) using the following instrument parameters: 80 kV, 80 μA, 0.4° rotation step, and trabecular bone parameters were measured, including bone mineral density (BMD) and bone morphometric parameters.
5
Bone Microarchitecture Analysis in Aging Mice
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To visualize the bone microarchitecture of the aging mice, femur samples fixed in 4% paraformaldehyde were analyzed by micro‐CT (General Electric, WI). The scanners were set at an isotropic resolution of 8 μm (energy: 80 kVp/80 μA; angle of increment: 0.5°; exposure time: 3000 ms/frame; and scanning time: 120 min) for the fixed bone samples. The built‐in software was acquired for data reconstruction. After scanning, a region of interest was selected 1 mm above the distal growth plate. Finally, the following parameters of the trabecular bone were calculated by the MicroView software: bone mineral density (BMD; g/cm3), bone volume/tissue volume (BV/TV; %), trabecular thickness (Tb.Th; mm), trabecular number (Tb.N; mm), and trabecular spacing (Tb.Sp; mm).
6
Micro-CT Analysis of Bone Graft Osteogenesis
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To assess the quality of the newly formed bone in the grafts, micro-CT was used (29 (link),30 (link)). At 8 weeks after implantation, all the extraneous vertebrae and soft tissues were dissected, and the implants were scanned using micro-CT (GE Healthcare, Canada) using the following parameters: 60 kV; 0.6 mm aluminum filter; 800 µA; number of players=150. More than 1,000 axial images were obtained from each graft at the threshold of 1,200 HU. The region of interest was chosen symmetrically in the left and right grafts as a cylinder (0.5x0.5x0.5 cm3) at rdifferent coronary positions. The grafts were equally portioned into five segments by 4 cross-sections. To evaluate osteogenesis, six morphometric indices were measured as follows: i) bone mineral density (BMD); ii) bone mineral content (BMC); iii) tissue mineral density (TMD); iv) tissue mineral content (TMC); v) bone volume fraction (BVF); vi) bone volume (BV) (30 (link),31 (link)). PHA containing no stem cells served as control. Two photographers analyzed the data in a blinded manner.
7
Micro-CT Analysis of Fracture Healing
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Micro-CT (GE Healthcare Biosciences) images were obtained using 80 kVp, 80 mA and 1100 ms exposure at a resolution of 45-micron voxel size for bone analysis. The individual scans were reconstructed and reoriented in a three-dimensional x, y and z plane, then several rotations and cropping of non-bone space were undertaken for uniformity. The region of interest was selected correlating to the region of fracture healing, 2 mm directly behind the third molar. Using Microview software the ROI was then highlighted and analyzed for bone mineral density (BMD), tissue mineral density (TMD) and bone volume fraction (BVF).
8
Micro-CT Tumor Volume Monitoring
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After inoculation, SCID mice were subjected to micro‐CT (GE Medical Systems, London, ON, Canada) examinations every week to monitor tumor growth. Scan parameters were as follows: isotropic voxel size, 45 μm; projections, 400; exposure time, 400 msec; voltage, 80 kW; and current, 450 μA. Tumor volume was determined by measuring the diameters of tumors in micro‐CT images and calculated by the equation V = 4/3π (1/4[D1 + D2])2, where D1 is the width of the tumor and D2 is the length of the tumor.
9
Thoracodorsal Kyphosis Morphometric Analysis
10
Micro-CT Analysis of Bone Microstructure
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For analysis of bone microstructure and mineralization, the left femurs were subjected to scanning using micro-CT (GE Healthcare, United States). After removing the soft tissues, the left femurs were placed with gauze in the sample holder and were scanned using micro-CT using a resolution of 6 μm, 80 kV, 80 μA, 400 number of views to obtain 3-dimensional images. The eXplore Reconstruction Utility software was used for three-dimensional reconstruction. Trabecular morphometry was characterized by measuring the volumetric parameters of bone mineral density (BMD), bone volume fraction (BVF), BS/BV, trabecular thickness (Tb.Th), trabecular number (Tb.N), and decreases in trabecular spacing (Tb.Sp) (Lontos et al., 2018 (link)).
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