Preparations were observed in a Zeiss Axioplan 2 microscope equipped with appropriate fluorescence filter sets. Black-and-white images were taken either with a cooled F-View CCD camera (DAPI- and CGH-stained preparations) or an Olympus CCD monochrome camera XM10, and captured separately for each fluorescent dye with either AnalySIS software, version 3.2 (Soft Imaging System GmbH) or with cellSens 1.9 digital imaging software (Olympus Europa Holding), respectively. The images were pseudo-coloured and merged using Adobe Photoshop CS4 and CS5 (Adobe Systems Inc.).
Analysis software version 3
Manufactured by Olympus
Sourced in Germany
AnalySIS software, version 3.2, is a data analysis and imaging solution developed by Olympus. It allows for the processing and analysis of digital microscopy images and data. The software provides basic image editing and measurement tools.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using analysis software version 3
1
Fluorescence Microscopy Image Acquisition
2
Fluorescence Microscopy Protocols for Dragonfly Cytogenetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rhionaeschna species preparations were observed in a Zeiss Axioplan 2 fluorescence microscope (Carl Zeiss, Jena, Germany). Black-and-white images were recorded with a cooled F-View CCD camera and captured with AnalySIS software, version 3.2 (Soft Imaging System GmbH, Münster, Germany). Preparations of A. cyanea were observed in a Leica DMLB fluorescence microscope equipped with a Leica DFC350 FX monochrome digital camera and Leica IM50 software, version 4.0 (Leica Microsystems Imaging Solutions Ltd., Cambridge, UK). Images of chromosomes were captured separately for each fluorescent dye and then pseudocolored (light blue for DAPI and red for Cy3) and superimposed using Adobe Photoshop, version 7.0 or Adobe Photoshop CS5.
3
Fluorescent Microscopy Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Preparations from FISH experiments were observed under a Zeiss Axioplan 2 microscope (Carl Zeiss Jena, Germany). Black-and-white images were recorded with a cooled F-View CCD camera using AnalySIS software, version 3.2 (Soft Imaging System GmbH, Münster, Germany). In all preparations, images were captured separately for each fluorescent dye, pseudocoloured (light blue for DAPI, green for fluorescein and red for Cy3) and superimposed with Adobe Photoshop, version 7.0.
4
Visualizing Au-NPs Encapsulation in Transporter Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, Au-NPs were employed as a representative drug model for encapsulation within TFs. The distinctive high contrast of Au-NPs against the background was utilised to clearly demonstrate their successful encapsulation within TFs. The morphologies of TF and TF-encapsulated Au-NPs were examined following negative staining using 2% uranyl acetate for 10 min. Transmission electron microscopy (TEM) (Philips CM12 TEM (FEI U. K. Ltd., Altrincham, UK) was used to investigate the morphology of the vesicles at 80 kV and images were captured with a Megaview III camera and AnalySIS software, version 3.2 (Soft Imaging System GmbH, Münster, Germany). TEM was performed at Central Biotechnology Services, Cardiff University.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!