Cells were lysed in a buffer containing 0.5% SDS, 0.1% v/v Triton X-100, 0.25% sodium deoxycholate, 12.5 mM tris, and 96 mM glycine (pH 8.3). Lysate protein concentrations were determined with a Bradford assay, and equal amounts of protein were run on SDS-PAGE gels for immunoblotting on PVDF membranes. Membranes were probed with antibodies specific for β-catenin (C7207, Sigma-Aldrich, St. Louis, MO, USA), histone 3 (H0164, Sigma-Aldrich), and β-actin (A5441, Sigma-Aldrich) before chemiluminescent imaging and quantitation with ImageJ.
C7207
Manufactured by Merck Group
Sourced in United States
C7207 is a laboratory equipment product offered by Merck Group. It is a device designed for laboratory use, but its core function is not provided in a detailed, unbiased, and factual manner without interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
H0164, by Merck Group (1 mentions)
A5441, by Merck Group (1 mentions)
Ab12221, by Abcam (1 mentions)
A11034, by Thermo Fisher Scientific (1 mentions)
Mab5428, by Merck Group (1 mentions)
T2762, by Thermo Fisher Scientific (1 mentions)
Ba 1000, by Vector Laboratories (1 mentions)
Ba 9200, by Vector Laboratories (1 mentions)
Phalloidin alexa488, by Thermo Fisher Scientific (1 mentions)
A 21271, by Thermo Fisher Scientific (1 mentions)
Rhodamin phalloidin, by Thermo Fisher Scientific (1 mentions)
L9393, by Merck Group (1 mentions)
Ab6586, by Abcam (1 mentions)
Gfp 1020, by Aves Labs (1 mentions)
Ab29112, by Abcam (1 mentions)
Ab24273, by Abcam (1 mentions)
Sc 8628, by Abcam (1 mentions)
Alexa fluor 555, by Thermo Fisher Scientific (1 mentions)
Ab13840, by Abcam (1 mentions)
Alexa fluor 594 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
6 protocols using c7207
1
Quantitative Immunoblotting of β-catenin
2
Antibody Profiling for Cell Signaling
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The primary antibodies used were rabbit polyclonal anti-phospho-ERK1/2 antibody (1:150; Phospho-p44/42 MAP kinase antibody, 9101S, Cell Signaling Technology, Danvers, MA, USA; [9 (link),10 (link)]), rabbit polyclonal anti-N-cadherin antibody (1/200; ab12221, Abcam, Cambridge, UK; [4 (link)], mouse monoclonal anti-β-catenin antibody (1:1000; C7207, Sigma-Aldrich; Saint Louis, MO, USA, [13 (link)]) and mouse monoclonal anti-RPE65 antibody (1:1000; MAB5428, Millipore; Billerica, MA, USA, [2 (link)]). The secondary antibodies used were biotinylated goat anti-rabbit IgG antibody (1:400; BA-1000, Vector Laboratories, Burlingame, CA, USA), biotinylated goat anti-mouse IgG antibody (1:400; BA-9200, Vector Laboratories, Burlingame, CA, USA), Alexa 488-conjugated goat anti-rabbit IgG antibody (1:500; A-11034, Thermo Fisher Scientific, Waltham, MA, USA) and tetramethylrhodamine-conjugated goat anti-mouse IgG antibody (1:200; T2762, Life Technologies, Rockville, MD, USA).
3
Comprehensive Immunostaining of Embryonic Tissues
4
SEM Sample Preparation Protocol
5
Pluripotency Marker Immunostaining Protocol
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Cells were fixed with paraformaldehyde, permeabilized, and stained with primary antibodies against OCT4 (Santa Cruz Biotechnology; sc-8628), NANOG (Abcam; ab808992), UTF1 (Abcam; ab24273), SALL4 (Abcam; ab29112), LIN28 (Abcam; ab46020), and CTNNB1 (Sigma-Aldrich; C7207) followed by staining with appropriate secondary antibodies conjugated to either Alexa Fluor 555 or 647 (Invitrogen). Images were acquired with a Neo 5.5 cSMOS camera (Andor) and processed and analyzed using NIS-Elements and Adobe Photoshop.
6
Immunohistochemical Analysis of Turtle Gonads
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Gonad-mesonephros complexes from turtle embryos of indicated stages were still immersed in 4% PFA overnight at 4°C, then moved through a MeOH gradient, embedded in paraffin wax and sectioned. Paraffin sections (~5 μm) were first deparaffinized, and antigens were unmasked by microwaving sections in 10 mM/L citrate buffer, pH 6.0 (15 minutes). Sections were covered with primary antibodies and incubated overnight at 4°C or 1 hour at room temperature. The primary antibodies used in this analysis included rabbit anti-Sox9 (AB5535, Chemicon, 1:1000), rabbit anti-Vasa (ab13840, Abcam, 1:200) and mouse anti-β-catenin (C7207, Sigma, 1:250). Secondary antibodies Alexa Fluor 594 donkey anti-rabbit IgG (A21207, Invitrogen) and Alexa Fluor 594 donkey anti-mouse IgG (A21203, Invitrogen), Alexa Fluor 488 donkey anti-rabbit IgG (A21206, Invitrogen), Alexa Fluor 488 donkey anti-mouse IgG (A21202, Invitrogen), which all diluted at 1:250, were used to detect primary antibodies. Nuclei were stained with DAPI. Gonad sections were imaged by confocal microscope (A1 Plus, Nikon).
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