Cell dyn 22

For blood analytics, two samples were taken, namely one in a 3-mL tube with ethylenediaminetetraacetic acid (EDTA) and another in a 3.5-mL tube with polyethene terephthalate (PET). Red blood cell count was carried out in an automated Cell-Dyn 3700 analyser (Abbott Diagnostics, Chicago, IL, USA) using internal (Cell-Dyn 22) and external (Program of Excellence for Medical Laboratories-PEML) controls. Values of erythrocytes, haemoglobin, haematocrit, and haematimetry indexes were determined. These data were used to verify the health status of the subjects and were not included in the study.

Venous blood samples were taken for general analytics, in one tube of 3 mL ethylenediaminetetraacetic acid (EDTA) for hemogram, and in another tube of 3.5 mL with polyethene terephthalate (PET) for biochemical parameters. Red blood cells count (RBC) was carried out in an automated Cell-Dyn 3700 analyzer (Abbott Diagnostics, Lake Forest IL, USA), using internal (Cell-Dyn 22, Abbott Diagnostics, IL, USA) and external (Program of Excellence for Medical Laboratories-PEML) controls. Values of erythrocytes, hemoglobin, haematocrit, and hematimetric indexes (mean cell volume, MCV; mean cell haemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; and red cell distribution width, RDW) were estimated.
Additionally, venous blood samples were collected pre VT1 test, post repeated sprint test, post second VT1 test, and 24 h after the end of the testing session for the measurement of antioxidant parameters (Figure 2). At each of the extraction points, 6 tubes of 3 mL of EDTA were obtained and one of them was centrifuged at 3500 rpm at 4 °C during 10 min and sent to the laboratory for later analysis. Urine samples, corresponding to 24 h urine collection from each participant after the supplementation, were frozen in liquid nitrogen after collection and thawed for its analysis.

Venous blood (arm antecubital area) was collected into one 3 mL ethylenediaminetetraacetic acid (EDTA) tube for hemogram and another 3.5 mL polyethene terephthalate (PET) tube was collected by a nurse for overall health analysis (visit 1). Red blood cell count was carried out in an automated Cell-Dyn 3700 analyzer (Abbott Diagnostics, Chicago, IL, USA) using internal (Cell-Dyn 22) and external (Program of Excellence for Medical Laboratories-PEML) controls. Values of erythrocytes, haemoglobin, haematocrit and haematimetric indexes were estimated.
Additionally, venous blood samples were collected in the baseline, after the maximum power stage (post-P_MAX) and during the resting phase (post-REC), to measure antioxidant and anti-inflammatory parameters (visits 3 and 5) (Figure 1). During every extraction point, 6 tubes of 3 mL of EDTA were obtained. Blood samples were centrifuged at 3500 rpm in 4 °C for 10 min and sent to the laboratory for later analysis.