Cells were prepared for flow cytometry as previously described [3 (link)] and the following antibodies were used: CD45-APC, CD15-BV605, CD33-PECy7 and Glycophorin A-PE (BD Biosciences, San Jose, CA, USA), CD33-BV421, CD19-PerCPCy5.5, CD14-BV605, CD117-PerCPCy5.5, CD3-PECy7 and FceRI-PE (BioLegend, San Diego, CA, USA).
Cd117 percpcy5
Manufactured by BioLegend
Sourced in United States
CD117-PerCPCy5.5 is a fluorophore-conjugated antibody that binds to the CD117 (c-kit) antigen. CD117 is a receptor tyrosine kinase that plays a role in cell signaling and is expressed on the surface of various cell types, including hematopoietic stem cells and mast cells. This product can be used in flow cytometry applications to identify and quantify CD117-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 apc, by BD (1 mentions)
Cd3 pe cy7, by BioLegend (1 mentions)
Cd33 bv421, by BioLegend (1 mentions)
Cd15 bv605, by BD (1 mentions)
Cd14 bv605, by BioLegend (1 mentions)
Fceri pe, by BioLegend (1 mentions)
Cd19 percp cy5, by BioLegend (1 mentions)
Cd33 pe cy7, by BD (1 mentions)
Glycophorin a pe, by BD (1 mentions)
Cd94 fitc, by BioLegend (1 mentions)
Anti nkg2a allophycocyanin z199 clone, by Beckman Coulter (1 mentions)
2 protocols using cd117 percpcy5
1
Multicolor Flow Cytometry Panel
2
Monoclonal Antibodies Characterization
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The following mAbs were isolated in our laboratory, licensed to the indicated companies and validated for their specificity: anti-NKp30 (AZ20, IgG1), anti-NKp44 (Z231, IgG1), anti-NKp46 (BAB281, IgG1) (Beckman Coulter/Immunotech, Marseille, France). The purified anti-PD-1 mAb (PD1.3.1.3 clone, IgG2b) was originally isolated at the Laboratoire Immunologie des Tumeurs, CRCM, Marseille-Luminy (France). 31 The following purchased mAbs were used in this study: Anti-CD56-PC7 (c218 clone), and anti-NKG2A allophycocyanin (Z199 clone) were purchased from Beckman Coulter/Immunotech (Marseille, France); anti-CD3-Viogreen (BW264/56 clone), anti-CD19-VioGreen (LT20 clone), anti-CD14-Viogreen (TÜK4 clone), anti-TNF-α-PE, anti-CD223-PE (LAG-3), mAbs were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); anti-TIM-3 was purchased from Novus Biologicals (Littleton, CO 80120, USA); anti IL-17A-FITC, CD45-APC-H7 (2D1 clone) from BD Bioscience; IL-22-PE TNF-α-eFluor450 purchased from eBioscience (San Diego, CA); CD94-FITC, CD127-BrilliantVio-let421, CD117-PerCP-Cy5.5, anti-CRTH2-APC, anti-TIGIT-PE purchased from BioLegend (San Diego, CA); IL-8-PE purchased from R&D (Minneapolis, MN) and anti-B7-H3 (D-8 clone, IgG2b) was purchased from Santa Cruz Biotechnology (Dallas, TX).
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