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Zymutest

Manufactured by Hyphen Biomed
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Sourced in France

Zymutest is a laboratory equipment product that serves as a technical tool for performing specific tests or analyses. It is designed to facilitate the measurement or assessment of various biological or chemical parameters within a controlled laboratory setting.

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Lab products found in correlation

Pai 1 antigen, by Hyphen Biomed (2 mentions) Imubind tissue factor elisa, by Sekisui (2 mentions) Thrombin reagent, by Siemens (1 mentions) Cs2100i, by Sysmex (1 mentions) Sta r, by Stago (1 mentions) Thromborel s, by Siemens (1 mentions) Bio plex protm human cytokine 27 plex assay, by Bio-Rad (1 mentions) Quantikine elisa, by R&D Systems (1 mentions)

Spelling variants of this product

Zymutest HIA IgG (5 citations) Zymutest PAI-1 Antigen (4 citations) Zymutest PAI-1 activity (3 citations) ZYMUTEST Free Protein S kit (2 citations) ZYMUTEST Factor IX ELISA kit (2 citations) Zymutest t-PA antigen (2 citations) Zymutest CRP (2 citations) Zymutest Activatable TAFI (2 citations) Zymutest D-dimer (2 citations)

7 protocols using zymutest

1

Secretion of ET-1, t-PA, and PAI-1 in NG and HG Cells

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Secretion of ET-1, t-PA and PA-1 by NGPC and HGPC exposed to NG, IG or HG medium (48 h) was measured by ELISA. For each ELISA, cell supernatants were prepared by seeding cells in triplicate in 96-well plates (2 × 104 cells/well) and exposing them to NG, IG or HG medium for 48 h under standard conditions. Medium from each triplicate was collected into a single tube and stored at − 80 °C for batch measurement. Samples were processed according to the manufacturer’s instructions for ET-1 (Abcam), t-PA antigen (Zymutest; Hyphen BioMed) and PAI-1 antigen (Zymutest; Hyphen BioMed), with absorbance measured at 450 nm using a Varioskan platereader with with SkanIt Software 2.4.5 (Varioskan Flash, Thermo).
Fiorello M.L., Treweeke A.T., Macfarlane D.P, & Megson I.L. (2020). The impact of glucose exposure on bioenergetics and function in a cultured endothelial cell model and the implications for cardiovascular health in diabetes. Scientific Reports, 10, 19547.
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2

Quantifying PAI-1 and t-PA Levels

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Plasminogen activator inhibitor type 1 (PAI-1 Antigen, Zymutest, HYPHEN BioMed), and tissue plasminogen activator (t-PA, Zymutest, HYPHEN BioMed) were determined by commercially available ELISA testing.
Marchetti M., Gomez-Rosas P., Sanga E., Gamba S., Verzeroli C., Russo L., Restuccia F., Schieppati F., Bonanomi E., Rizzi M., Fagiuoli S., D'Alessio A., Lorini L, & Falanga A. (2021). Endothelium Activation Markers in Severe Hospitalized COVID-19 Patients: Role in Mortality Risk Prediction. TH Open: Companion Journal to Thrombosis and Haemostasis, 5(3), e253-e263.
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3

Quantification of PAI-1 and t-PA in Plasma

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Plasminogen activator inhibitor type 1 (PAI-1 Antigen, Zymutest, Hyphen BioMed), and tissue plasminogen activator (t-PA, Zymutest, Hyphen BioMed) were determined in plasma samples by commercially available ELISA.
Marchetti M., Gomez-Rosas P., Russo L., Gamba S., Sanga E., Verzeroli C., Ambaglio C., Schieppati F., Restuccia F., Bonanomi E., Rizzi M., Fagiuoli S., D’Alessio A., Gerotziafas G.T., Lorini L, & Falanga A. (2022). Fibrinolytic Proteins and Factor XIII as Predictors of Thrombotic and Hemorrhagic Complications in Hospitalized COVID-19 Patients. Frontiers in Cardiovascular Medicine, 9, 896362.
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4

Comprehensive Coagulation Factor Analysis

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FII, FV, FVII, FX (Neoplastine R), FVIII, FIX, FXI, FXII (CK Prest) activity levels were measured by clotting assays using specific factor‐depleted plasma as substrate on Sta‐R (Stago, Paris, France). Fibrinogen level (Clauss method, Thrombin Reagent; Siemens, Marburg, Germany), FXIII activity and vWF antigen and activity (FXIIIact/subs, vWF Reag and vWF Ac Reagens; Siemens) measurements were performed on a Sysmex CS2100i. α2‐antiplasmin level was measured using a chromogenic assay (Stachrom; Stago), tissue plasminogen activator (tPA) and plasminogen activator inhibitor 1 (PAI‐1) activity were measured using bio‐immunoassays (Zymutest, Hyphen‐BioMed, Neuville‐sur‐Oise, France).
Vries M.J., van der Meijden P.E., Kuiper G.J., Nelemans P.J., Wetzels R.J., van Oerle R.G., Lancé M.D., ten Cate H, & Henskens Y.M. (2018). Preoperative screening for bleeding disorders: A comprehensive laboratory assessment of clinical practice. Research and Practice in Thrombosis and Haemostasis, 2(4), 767-777.
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5

Comprehensive Hematological and Coagulation Profiling

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The following tests were done on all patients and controls: Complete blood counts (Automated hematology analyser LH500), biochemical parameters including serum calcium, creatinine and total proteins, β-2 microglobulin, hemostatic parameters including prothrombin time (PT; Dade Behring Thromborel S), activated partial thromboplastin time (APTT; Dade Behring Actin FS), thrombin time (TT; Sigma- Aldrich), plasma fibrinogen (Quantia, Tulip), factor VIII assay (PZ Cormay S.A.), D-dimer (Zymutest, Hyphen Biomed) and LA (dRVVT screening and confirmatory test, Tulip).
Gogia A., Sikka M., Sharma S, & Rusia U. (2018). Hemostatic Abnormalities in Multiple Myeloma Patients. Asian Pacific Journal of Cancer Prevention : APJCP, 19(1), 127-130.
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6

Multiplex Cytokine Profiling in Research

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The Bio-Plex ProTM human cytokine 27-plex assay (Bio-Rad, Vienna, Austria) was used to quantify interleukin (IL)-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, and IL-1 receptor antagonist (IL-1ra); basic fibroblast growth factor (bFGF); granulocyte colony-stimulating factor (G-CSF); granulocyte-macrophage colony-stimulating factor (GM-CSF); interferon-gamma (IFN-γ); the chemokines CXCL10 (IP-10, interferon-inducible protein 10), CCL2 (MCP-1, monocyte chemotactic protein-1), CCL3 and CCL4 (MIP-1α and MIP-1β, macrophage inflammatory protein-1 alpha and beta); CCL5 (RANTES, regulated on activation, normal T-cell expressed and secreted); CCL11 (eotaxin, eosinophil chemotactic protein); platelet-derived growth factor (PDGF); tumor necrosis factor-alpha (TNF-α); as well as vascular endothelial growth factor (VEGF). Selected plasma samples were further characterized with a membrane-based antibody array (Proteome ProfilerTM ARY002, R&D Systems, Minneapolis, USA) detecting 102 cytokines, chemokines, and growth factors. Plasminogen activator inhibitor-1 (PAI-1) and human endothelial cell-specific molecule-1 (ESM-1, endocan) were quantified by enzyme-linked immunosorbent assays (Zymutest, Hyphen BioMed, Neuville-sur-Oise, France, and Lunginnov, Lille, France, respectively).
Eichhorn T., Rauscher S., Hammer C., Gröger M., Fischer M.B, & Weber V. (2016). Polystyrene-Divinylbenzene-Based Adsorbents Reduce Endothelial Activation and Monocyte Adhesion Under Septic Conditions in a Pore Size-Dependent Manner. Inflammation, 39(5), 1737-1746.
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7

ELISA Tissue Factor Quantification

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We carried out ELISA according to the manufacturers’ protocols. The kits used in this study included the Imubind Tissue Factor ELISA (Sekisui Diagnostics), Zymutest (Hyphen BioMed), CD142 ELISA (Abcam), and Quantikine ELISA (R&D Systems).
Kobayashi S., Koizume S., Takahashi T., Ueno M., Oishi R., Nagashima S., Sano Y., Fukushima T., Tezuka S., Morimoto M., Nakamura S., Narimatsu H., Ruf W, & Miyagi Y. (2021). Tissue factor and its procoagulant activity on cancer‐associated thromboembolism in pancreatic cancer. Cancer Science, 112(11), 4679-4691.
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