Cultures were grown over night in MMB medium. The next day, 10 mM 2-BE were added and the cultures were incubated for another 3 h. Cm was added to stop protein synthesis and the cultures were incubated overnight. The medium was centrifuged at 5000×g for 15 min at room temperature to remove cells. The supernatant was acidified with phosphoric acid to pH 2 and extracted with an equal volume of DCM for 3 min. Extracts were dried over anhydrous sodium sulfate and analyzed by GC–MS [Agilent Technologies; 7890A GC system, 5975C VL MSD detector, 7683B injector, VF-5 ms column (60 m × 0,25 mm × 0,25 µm)]. The temperature program was as follows: hold for 1 min at 40 °C, increase to 200 °C at 10 °C min−1. 1 µl was injected and the sample was analyzed in split mode (1:10). Helium was used as carrier gas at a flow rate of 0.927 ml min−1. The goal was to detect potential metabolites qualitatively, not quantitatively. Therefore, the creation of calibration curves was omitted.
5975c vl msd detector
Manufactured by Agilent Technologies
The Agilent 5975C VL MSD detector is a high-performance gas chromatography-mass spectrometry (GC-MS) system. It is designed to accurately detect and identify a wide range of organic compounds in complex samples. The core function of the 5975C VL MSD is to provide sensitive and reliable mass spectrometry analysis for various applications.
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Lab products found in correlation
2 protocols using 5975c vl msd detector
1
Detection of Metabolite Production
2
Bioremediation of Contaminated Oil by GC-MS
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The culture was then separated from the soil media by dissolving it in 10 mL organic solvent n-hexane using a centrifuge at 2000 rpm for 10 minutes.29 (link) The resulting oil was dried using an oven at 70 °C for 10 min to determine the mass of the bioremediated oil and obtain the TPH value by gravimetric method.30 (link) Degradation results from oil bioremediation process were identified by analyzing oil samples. Analysis was carried out using a hydrocarbon profile test with a Gas Chromatography-Mass Spectrometry (GC-MS) instrument. The oil samples obtained were diluted 20× using n-hexane before being analyzed. GCMS was performed using an Agilent Technologies 7890 GC System linked to an Agilent Technologies 5975C VL MSD Detector with a 30 m × 50 μm × 0.25 μm Agilent 19091S-433 column. The oven temperature was programmed to start from 80 °C and held for 2 min, and then the temperature was increased to 280 °C at 5 °C min−1 and held for 15 min greater.13
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