Particle sizing was carried out using dynamic light scattering with a Zetasizer Nano ZS (Malvern Instruments Ltd., Worcestershire, UK). For each measurement, the sample suspension without further treatment (~1.0 mL) was put in a disposable polystyrene cuvette (SARSTEDT AG & Co., Nümbrecht, Germany). The nanocarrier surface ζ-potential was measured using a Zetasizer Nano ZS by using disposable folded capillary cells (DTS1070, Malvern Instruments, Malvern, UK). Both the size and ζ-potential were recorded as the average of three measurements. The structure of the fabricated nanoparticle was evaluated by transmission electron microscopy (TEM; JEOL 1400 Plus, JEOL, Tokyo, Japan) at an acceleration voltage of 120 kV. The TEM samples were prepared by depositing 10 µL of the NP suspensions (~1.0 mg/mL) onto carbon-coated copper grids (200 mesh; Ted Pella, Inc., Redding, CA, USA). Samples were blotted away after 5 min incubation then air-dried prior to imaging.
Disposable polystyrene cuvette
Manufactured by Sarstedt
Sourced in Germany
The Disposable polystyrene cuvette is a single-use laboratory equipment item used for spectrophotometric analyses. It is made of clear polystyrene material and designed to hold liquid samples for measurement of absorbance or transmittance.
Automatically generated - may contain errors
3 protocols using disposable polystyrene cuvette
1
Characterization of Nanoparticle Formulations
2
Nanovaccine Characterization by DLS and ELS
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The hydrodynamic
radius and the surface charge of the nanovaccine were evaluated by
DLS and ELS with a Zetasizer NanoZS (Malvern Instruments Ltd., UK).
Each sample was diluted 1:50, and 1 mL of the dilution was pipetted
in a disposable polystyrene cuvette (Sarstedt AG&Co., Germany)
to determine the size. The temperature of each sample was equilibrated
to 25 °C before each measurement. About 750 μL of the particle
suspension in Milli Q-water (pH 7.4) was pipetted into a disposable
folded capillary cell (DTS1070, Malvern Ltd., UK).
radius and the surface charge of the nanovaccine were evaluated by
DLS and ELS with a Zetasizer NanoZS (Malvern Instruments Ltd., UK).
Each sample was diluted 1:50, and 1 mL of the dilution was pipetted
in a disposable polystyrene cuvette (Sarstedt AG&Co., Germany)
to determine the size. The temperature of each sample was equilibrated
to 25 °C before each measurement. About 750 μL of the particle
suspension in Milli Q-water (pH 7.4) was pipetted into a disposable
folded capillary cell (DTS1070, Malvern Ltd., UK).
3
Nanoparticle Characterization by Dynamic Light Scattering
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After preparation, the nanoparticles were characterized by dynamic light scattering to obtain information about their size, polydispersity index (PDI), and zeta (ζ)-potential, using Malvern Zetasizer Nano instrument (Malvern Ltd., UK). About 1 mL of a 1:100 dilutions of each sample with demineralized water, was pipetted into a disposable polystyrene cuvette (Sarstedt AG & Co., Germany) and the measurements were carried out at 25 ± 0.1 • C. The surface ζ-potential of the nanoparticles was measured by pipetting 750 µL of each particle suspension into a disposable folder capillary cell (DTS1070, Malvern, UK); all samples were redispersed in MillQ-water (pH 7.4) before assessing their surface potential. The results are shown as numeric average and standard deviation of the measurements of 3 different samples, each sample measured 3 times.
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