Human buffy coats

Manufactured by GE Healthcare

Sourced in United States

Human buffy coats are a component of whole blood that is separated through centrifugation. The buffy coat contains a concentrated layer of white blood cells and platelets. This product can be used for various laboratory applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by American Type Culture Collection (2 mentions) Unlabeled oxldl, by Biomedical Technologies (2 mentions) Ficoll paque premium, by GE Healthcare (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) Percoll, by GE Healthcare (1 mentions) Macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions) Ficoll paque premium 1.077 g ml, by GE Healthcare (1 mentions) Human serum, by MP Biomedicals (1 mentions)

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Buffy coat (7 citations)

2 protocols using human buffy coats

Synthesis and Characterization of PEGylated Nano-Conjugates

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All reagents and solvents were purchased from Sigma-Aldrich (Milwaukee, WI) and were used directly as received unless otherwise noted. Anhydrous dimethylformamide (DMF) was further dried over 4 Å molecular sieves overnight before use. Hydrochloric acid (HCl, 1 N), polytetrafluoroethylene (PTFE) syringe filters, and poly(vinylidene fluoride) (PVDF) syringe filters were purchased from Fisher Scientific (Fair Lawn, NJ). Di-tert-butyl L-tartrate [25 ] and dimethyl amino pyridine p-toluene sulphonate (DPTS) [26 ] were prepared as previously published. Monomethoxy-poly(ethylene glycol) (PEG, 5 kDa) was azeotropically distilled with toluene (3× 50 mL) prior to use. For cell experiments, reagents include human buffy coats purchased from the Blood Center of New Jersey (East Orange, NJ) and New York Blood Center (Long Island City, NY), 1.077 g/cm³ Ficoll-Paque Premium purchased from GE Healthcare (Pittsburgh, PA), RPMI 1640 from ATCC (Manassas, VA), macrophage colony stimulating factor (M-CSF) from PeproTech (Rocky Hill, NJ), fetal bovine serum (FBS) from Life Technologies (Grand Island, NY), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) labeled oxLDL from Kalen Biomedical (Montgomery Village, MD), and unlabeled oxLDL from Biomedical Technologies Inc. (Stoughton, MA).

Zhang Y., Li Q., Welsh W.J., Moghe P.V, & Uhrich K.E. (2016). Micellar and Structural Stability of Nanoscale Amphiphilic Polymers: Implications for Anti-atherosclerotic Bioactivity. Biomaterials, 84, 230-240.

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Synthesis and Characterization of Nanoparticles

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All reagents and materials were purchased from Sigma-Aldrich (Milwaukee, WI, USA) and were used as received unless noted otherwise. Hydrochloric acid (HCl, 1 N) was purchased from Fisher Scientific (Fair Lawn, NJ, USA), and silica from VWR (Radnor, PA, USA). The confined impinging jet mixer was provided by Prof. Robert Prud’homme at Princeton University (Princeton, NJ, USA). Cell culture assays used human buffy coats purchased from the New York Blood Center (New York, NY, USA) or New Jersey Blood Center (East Orange, NJ, USA), Ficoll-Paque premium 1.077 g/mL and Percoll 1.3 g/mL from GE Healthcare (Fairfield, CT, USA), FEP Teflon cell culture bags from Cellgenix, RPMI-1640 from ATCC (Manassas, VA, USA), macrophage colony stimulating factor (MCSF) from PeproTech (Rocky Hill, NJ, USA), penicillin/streptomycin from Lonza (Basel, Switzerland), fetal bovine serum (FBS) from Life Technologies (Carlsbad, CA, USA), human serum from MP Biomedical, unlabeled oxLDL from Biomedical Technologies Inc. (Ward Hill, MA, USA), and 3,3′-dioctadecyloxacarbocyanine (DiO) labeled oxLDL from Kalen Biomedical (Montgomery Village, MD, USA).

Moretti A., Li Q., Chmielowski R., Joseph L.B., Moghe P.V, & Uhrich K.E. (2018). Nanotherapeutics Containing Lithocholic Acid-Based Amphiphilic Scorpion-Like Macromolecules Reduce In Vitro Inflammation in Macrophages: Implications for Atherosclerosis. Nanomaterials, 8(2), 84.

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