S2824

Manufactured by Selleck Chemicals

S2824 is a laboratory equipment product. It is designed for use in scientific research and analysis applications.

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Lab products found in correlation

Rapamycin, by Cell Signaling Technology (1 mentions) Trypsin, by neoFroxx (1 mentions) Cell incubator, by Thermo Fisher Scientific (1 mentions) Tunel reaction buffer, by Roche (1 mentions) Eclipse ti e inverted microscope, by Nikon (1 mentions) H2o2 solution, by Merck Group (1 mentions) Triton x 100, by Solarbio (1 mentions)

2 protocols using s2824

Irradiation and Cytokine Secretion Assay

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For TPCA-1 treatment, cells were detached using EDTA, irradiated using a ¹³⁷Cs source, seeded in 25-cm² tissue culture flasks, and incubated at 37°C 5% CO₂. TPCA-1 (1 or 10 μM; S2824, Selleckchem) was added to cell cultures after irradiation.
For rapamycin treatment, cells were passaged, placed into growth medium containing 5 nM of rapamycin (9904S, Cell Signaling Technology), and incubated at 37°C 5% CO₂ for 1 day. Cells were detached and irradiated as indicated above, placed in 25-cm² tissue culture flasks in growth media containing 5 nM rapamycin, and incubated at 37°C 5% CO₂.
On day 2 after irradiation, cells were placed in serum-free, TPCA-1/rapamycin-free growth media containing 0.5% bovine serum albumin (BSA). Cell-free supernatants were harvested on day 3 after irradiation and used for migration assays or enzyme-linked immunosorbent assay (ELISA).

Walle T., Kraske J.A., Liao B., Lenoir B., Timke C., von Bohlen und Halbach E., Tran F., Griebel P., Albrecht D., Ahmed A., Suarez-Carmona M., Jiménez-Sánchez A., Beikert T., Tietz-Dahlfuß A., Menevse A.N., Schmidt G., Brom M., Pahl J.H., Antonopoulos W., Miller M., Perez R.L., Bestvater F., Giese N.A., Beckhove P., Rosenstiel P., Jäger D., Strobel O., Pe’er D., Halama N., Debus J., Cerwenka A, & Huber P.E. (2022). Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8. Science Advances, 8(12), eabh4050.

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Apoptosis Assessment of RPTC Cells

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The cells with a confluence of up to 80% were extracted from the cell incubator, namely RPTC HNF‐1 NC cells and RPTC HNF‐1 KD cells. The cells were digested by trypsin (1004GR025, BioFroxx, Germany) and collected. The cells were evenly spread to the 24‐well plate according to the density of 8 × 104/well and then cultured for 24 hours in the cell incubator (Model: 3111; Thermo Scientific, USA) at 37℃ and 5% CO₂. The next day, the cells were grouped as follows: NC or KD cells treated with or without 20 μmol/L cisplatin, NC or KD cells treated with cisplatin and TPCA‐1 (S2824; Selleck Chemicals), and NC or KD cells treated with TPCA‐1 only. Cells were rinsed with PBS for 2‐3 times and fixed at room temperature by 4% paraformaldehyde for 1 hour. After washed with PBS, cells were blocked with 3% H₂O₂ solution (323381; Sigma) at room temperature away from light for 10 minutes. Cells were permeabilized with Triton X‐100 (T8200; Solarbio) at concentration of 0.05% (v/v) for 2 minutes on ice and incubated with TUNEL reaction buffer (P48307791‐131; Roche) at 37℃ for 1 hour away from light. The cell nuclei were stained with Hoechst 33342 (10 μg/mL) for 2 minutes at room temperature. The cells were observed under a Nikon Inverted Microscope Eclipse Ti‐E (Nikon, Japan).

Zhang Y., Hao J., Du Z., Li P., Hu J., Ruan M., Li S., Ma Y, & Lou Q. (2021). Inhibition of hepatocyte nuclear factor 1β contributes to cisplatin nephrotoxicity via regulation of nf‐κb pathway. Journal of Cellular and Molecular Medicine, 25(6), 2861-2871.

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