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Anti car apc antibody

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The Anti-CAR-APC antibody is a laboratory reagent used in flow cytometry and other immunoassays. It is designed to detect the presence and expression of the Chimeric Antigen Receptor (CAR) on the surface of cells.

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Lab products found in correlation

Normal mouse igg, by Thermo Fisher Scientific (2 mentions) Cd3 apc cy7 ucht1, by Thermo Fisher Scientific (2 mentions) Cd4 fitc rpa t4, by BD (2 mentions) Cd8 pecy7 rpa t8, by BD (2 mentions) 7 aminoactinomycin d 7 aad, by BD (2 mentions)

2 protocols using anti car apc antibody

1

Characterizing CAR T-cell Persistence

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These methods apply to the data shown in Figure 2j–k. Cryopreserved patient peripheral blood mononuclear cells (PBMCs) collected one month after CAR T-cell infusion were thawed and then washed once with FACS buffer containing 0.4% BSA and 0.1% weight/volume sodium azide in PBS. Samples were Fc blocked with normal mouse IgG (Invitrogen) and stained with CD3-APC Cy7 (UCHT1, eBioscience), CD4 FITC (RPA-T4, BD), CD8 PECy7 (RPA-T8, BD), 7-amino actinomycin D (7AAD, BD), and anti-CAR-APC antibody (the anti-CAR antibody was generated by Kite Pharma, Inc.). The anti-CAR antibody bound equivalently to the linker in the scFv of both Hu19-CD828Z and FMC63–28Z (Supplementary Figure 3)35 . Samples were acquired using a BD LSR II with Diva software and data was analyzed using FlowJo version 10. The gating strategy is shown in Supplementary Figure 7.
Brudno J.N., Lam N., Vanasse D., Yueh-wei S., Rose J.J., Rossi J., Xue A., Bot A., Scholler N., Mikkilineni L., Roschewski M., Dean R., Cachau R., Youkharibache P., Patel R., Hansen B., Stroncek D.F., Rosenberg S.A., Gress R.E, & Kochenderfer J.N. (2020). Safety and feasibility of anti-CD19 CAR T cells with fully-human binding domains in patients with B-cell lymphoma. Nature medicine, 26(2), 270-280.
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2

Characterizing CAR T-cell Persistence

Check if the same lab product or an alternative is used in the 5 most similar protocols
These methods apply to the data shown in Figure 2j–k. Cryopreserved patient peripheral blood mononuclear cells (PBMCs) collected one month after CAR T-cell infusion were thawed and then washed once with FACS buffer containing 0.4% BSA and 0.1% weight/volume sodium azide in PBS. Samples were Fc blocked with normal mouse IgG (Invitrogen) and stained with CD3-APC Cy7 (UCHT1, eBioscience), CD4 FITC (RPA-T4, BD), CD8 PECy7 (RPA-T8, BD), 7-amino actinomycin D (7AAD, BD), and anti-CAR-APC antibody (the anti-CAR antibody was generated by Kite Pharma, Inc.). The anti-CAR antibody bound equivalently to the linker in the scFv of both Hu19-CD828Z and FMC63–28Z (Supplementary Figure 3)35 . Samples were acquired using a BD LSR II with Diva software and data was analyzed using FlowJo version 10. The gating strategy is shown in Supplementary Figure 7.
Brudno J.N., Lam N., Vanasse D., Yueh-wei S., Rose J.J., Rossi J., Xue A., Bot A., Scholler N., Mikkilineni L., Roschewski M., Dean R., Cachau R., Youkharibache P., Patel R., Hansen B., Stroncek D.F., Rosenberg S.A., Gress R.E, & Kochenderfer J.N. (2020). Safety and feasibility of anti-CD19 CAR T cells with fully-human binding domains in patients with B-cell lymphoma. Nature medicine, 26(2), 270-280.
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