Planneofluar z 1x 0.25 objective

ctnnd1 embryos were photographed at approximately the 6-somite stage using identical magnification and lighting settings across embryos on a Zeiss AXIO Zoom V16 microscope fitted with a PlanNeoFluar Z 1x/0.25 objective and Axiocam 503 color camera. Embryo photographs were color-balanced using the BIOP SimpleColorBalance plugin in ImageJ (Schindelin et al., 2012 (link)). The time-lapse recording of developing ctnnd1 embryos was made from approximately the 4–6 somite stage to the 12–14 somite stage using the “TIME-LAPSE” function on an iPhone 8 mounted to a Zeiss Stemi 2000 stereo microscope with a Gosky Universal Cell Phone Adapter Mount.

Zebrafish embryos at 1 dpf were anesthetized, mounted in 2% methylcellulose in embryo medium and imaged on an AxioZoom V16 (Zeiss) microscope with a PlanNeoFluar Z 1x/0.25 objective. For time-lapse imaging, ten embryos were placed in embryo medium without methylene blue. The embryos were imaged for 20 h every 10 min on an ACQUIFER Imaging Machine (DITABIS AG) with the settings described in Čapek et al. [3 (link)].