For retrograde tracer injections into IC and SC, 80 nl of fluorescently conjugated Cholera toxin (CTb 555 or 647, 0.25%; Invitrogen) was injected into each location through a pulled glass micropipette using the pressure injection method49 (link). Following 5–7 days, animals were deeply anesthetized and transcardially perfused with 4% paraformaldehyde. Brain tissue was sliced into 150 μm sections using a vibratome and sections were mounted onto glass slides and imaged using a confocal microscope.
Cholera toxin ctb 555 or 647
Manufactured by Thermo Fisher Scientific
Cholera toxin (CTb 555 or 647) is a fluorescently labeled bacterial protein used in laboratory research. It functions as a cell-tracing agent, allowing researchers to visualize and track the movement and distribution of specific cell types or structures within a sample.
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Lab products found in correlation
2 protocols using cholera toxin ctb 555 or 647
1
Retrograde Tracer Injections for Neural Mapping
2
Retrograde Tracer Injections into IC and SC
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For retrograde tracer injections into IC and SC, 80 nl of fluorescently conjugated Cholera toxin (CTb 555 or 647, 0.25%; Invitrogen) was injected into each location through a pulled glass micropipette using the pressure injection method49 (link). Following 5–7 days, animals were deeply anaesthetized and transcardially perfused with 4% paraformaldehyde. Brain tissue was sliced into 150-μm sections using a vibratome and sections were mounted onto glass slides and imaged using a confocal microscope.
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