Collected tissues were lysed and isolated total RNA with TRIZOL (Invitrogen, USA) according to the protocols. cDNA was generated with the miScript reverse transcription kit (Qiagen) and PCR was executed with the SYBR PrimeScript TM miRNA RT-PCT kit (Takara). Cel-miR-39 was used as the internal standard to assess the expression level of miR-1306 with the 2− ΔΔCT method.
Sybr primescript mirna rt pct kit
Manufactured by Takara Bio
Sourced in Japan
The SYBR PrimeScript TM miRNA RT-PCT kit is a reagent kit designed for the reverse transcription and real-time PCR detection of microRNA (miRNA) expression. The kit includes all the necessary components for the RT-PCR process, including reverse transcriptase enzyme, SYBR Green master mix, and miRNA-specific primers.
Automatically generated - may contain errors
Lab products found in correlation
Miscript reverse transcription kit, by Qiagen (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Mir x mirna first strand synthesis kit, by Takara Bio (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
2 protocols using sybr primescript mirna rt pct kit
1
RNA Extraction and miRNA Quantification
2
Quantification of lncRNA and miRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole RNA was extracted from the treated cells with TRIzol reagent (Invitrogen, USA). PrimeScript RT Reagent Kit was applied to reverse transcribed mRNA into cDNA and SYBR Premix Ex Taq II (TaKaRa, Japan) was used to perform qRT-PCR. GAPDH was served as an internal standard for detection of BCYRN1 and POU3F2. miRNA was reverse transcribed into cDNA by Mir-X™ miRNA First Strand Synthesis Kit and qRT-PCR was executed by SYBR PrimeScriptTM miRNA RT-PCT Kit (TaKaRa, Japan). U6 was regarded as an internal standard for detection of miR-490-3p. 2 − ΔΔCT method was employed to assess the relative expression of BCYRN1, miR-490-3p, and POU3F2.
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