Production or consumption of glucose, fructose, glycerol, acetic acid and ethanol, were determined by HPLC in duplicate, using a Surveyor Plus chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively). A Hyper REZ XP carbohydrate H+ 8 μm column and guard (Thermo Fisher Scientific) were used and maintained at 50 °C. Elution was performed with 1.5 mM H2SO4 as mobile phase, at a flow rate of 0.6 mL/min. Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
Surveyor pda plus
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Surveyor PDA Plus is a portable data acquisition (PDA) device designed for laboratory applications. It provides real-time monitoring and data collection capabilities for various laboratory instruments and processes. The core function of the Surveyor PDA Plus is to acquire and record data from connected devices, enabling users to track and analyze experimental or operational parameters.
Automatically generated - may contain errors
Lab products found in correlation
Surveyor ri plus, by Thermo Fisher Scientific (4 mentions)
Hyper rez xp carbohydrate h 8 μm column and guard, by Thermo Fisher Scientific (2 mentions)
Surveyor plus chromatograph, by Thermo Fisher Scientific (1 mentions)
Surveyor plus liquid chromatograph, by Thermo Fisher Scientific (1 mentions)
Hyperrez xp carbohydrate h, by Thermo Fisher Scientific (1 mentions)
4 protocols using surveyor pda plus
1
HPLC Analysis of Metabolites
2
HPLC Metabolite Quantification Protocol
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Concentration of the main metabolites, glucose, fructose, glycerol, acetic acid and ethanol, was determined in duplicate for each sample, by HPLC, using a Surveyor Plus chromatograph (Thermo Fisher Scientific, Waltham, MA, United States) equipped with a refraction index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively). HI-Plex H 300 mm × 7.7 mm column and guard (Agilent Technologies) were used and maintained at 50°C. Elution was performed with 1.5 mM H2SO4 as mobile phase, at 0.6 mL/min. Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
3
Quantitative Analysis of Fermentation Metabolites
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Production and consumption of the main fermentation-related metabolites in daily samples, (glucose, fructose, glycerol, acetic acid and ethanol) were determined in duplicate using a Surveyor Plus chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a refractive index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively). Hyper REZ XP carbohydrate H+8 μm column and guard (Thermo Fisher Scientific) were used and maintained at 50°C. Elution was performed with 1.5 mM H 2 SO 4 as mobile phase, at a flow rate of 0.6 mL/min.
Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
One way analysis of variance was carried out on the main fermentation metabolites. Means of biological replicates were compared using Tukey's test, with significance level set at 5%. All analyses were performed using SPSS Statistics v. 23 program (IBM, Armonk, NY).
Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
One way analysis of variance was carried out on the main fermentation metabolites. Means of biological replicates were compared using Tukey's test, with significance level set at 5%. All analyses were performed using SPSS Statistics v. 23 program (IBM, Armonk, NY).
4
Quantifying Metabolite Production in Cultures
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Production and consumption of the main metabolites, glucose, fructose, glycerol, and ethanol, were determined in duplicate using a Surveyor Plus Liquid Chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively) on a 300 × 7.7 mm HyperREZ TM XP Carbohydrate H+ (8 μm particle size) column and guard (Thermo Fisher Scientific). The column was maintained at 50 °C, and 1.5 mM H 2 SO 4 was used as the mobile phase at a flow rate of 0.6 mL/min. Prior to injection in duplicate, the samples were filtered through 0.22 μm pore size nylon filters (Micron Analitica, Madrid, Spain) and diluted 10-fold in MilliQ water.
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