Human gastric cancer cell lines (SNU-638, MKN1 and AGS) were obtained from the Cell Resource Center (Chinese Academy of Sciences, Shanghai, China). SNU-638 and MKN1 cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc.) and AGS cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc.). Cells were cultured at 37°C in a humidified atmosphere containing 5% CO2. Tan IIA was obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany; purity, ≥97%) and dissolved in dimethyl sulfoxide (DMSO) to make 2.5 µg/ml stock solutions. Gastric cancer cells were treated with Tan IIA in different times (0, 12, 24, 48 and 72 h) or concentrations (0, 2.5, 5 and 10 µg/ml) at 37°C in humidified air and 5% CO2 atmosphere.
Snu 638
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SNU-638 is a laboratory equipment designed for various scientific applications. It features a compact and durable construction, suitable for use in a range of laboratory settings. The core function of the SNU-638 is to provide a reliable and consistent performance for the intended tasks.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Snu 638, by Korean Cell Line Bank (3 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Snu 216, by Thermo Fisher Scientific (2 mentions)
Snu 620, by Thermo Fisher Scientific (2 mentions)
Snu 668, by Thermo Fisher Scientific (2 mentions)
Nugc 3, by Thermo Fisher Scientific (2 mentions)
Nci n87, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Tan iia, by Merck Group (1 mentions)
Hek293, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Mrc 5, by Thermo Fisher Scientific (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Sk hep1, by Thermo Fisher Scientific (1 mentions)
7 protocols using snu 638
1
Tan IIA Inhibits Gastric Cancer Cells
2
Cell Culture Conditions for Various Human Cell Lines
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Human embryonic kidney cells (HEK293), human normal lung fibroblast cell (MRC-5), human colorectal cancer cells (HCT116), human breast cancer cells (MDA-MB-231), human lung cancer cells (A549), human liver cancer cells (SK-HEP-1), and human gastric cancer cells (SNU-638) were purchased from the American Type Culture Collection (Manassas, VA, USA). HEK293, MRC5, MDA-MB-231 and SK-HEP-1 were maintained in DMEM, while HCT116, A549 and SNU-638 were cultured in RPMI-1640 media supplemented with 10% FBS and antibiotics-antimycotics (PSF: 100 units/mL sodium penicillin G, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B) (Thermo-Scientific, MA, USA) in a humidified incubator containing 5% CO2 at 37°C.
3
Stomach Cancer Cell Line Cultivation
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The Stomach cancer cell lines AGS, SNU-216, NCI-N87, SNU-620, SNU-638, SNU-668, and NUGC-3 were purchased from the Korean Cell Line Bank (Cancer Research Center, Seoul National University, Seoul, Korea)and grown in RPMI1640 medium (Gibco) supplemented with 5% fetal bovine serum (Gibco) and 100 μg/ml antibiotics (100 U/ml penicillin and 100 μg/ml streptomyhcin, Gibco). Cells (1x105 cells/well) were plated in 24-well cell culture plates and grown at 37°C in a humidified, 5% CO2/air atmosphere. Cell viability assayed by WST-1. Please see other experimental procedures in Supplemental Experimental Procedures.
4
GC Cell Line Culturing and Signaling
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The GC cell lines SNU484 and SNU638 were purchased from the Korean Cell Line Bank (Seoul National University, Seoul, South Korea). SNU484 and SNU638 cells were cultured in RPMI 1640 (Gibco, Grand Island, NY, USA) medium supplemented with 10% fetal bovine serum (WELGENE, Gyeongsan-si, South Korea) and 1% penicillin–streptomycin (SIGMA, St. Louis, MO, USA) in a humidified incubator maintained at 37 ℃ with 5% CO2. All experiments were performed with cells having ~70–90% confluency. Panobinostat was purchased from ChemCruz from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). LY294002 was procured from Sigma-Aldrich (St. Louis, MO, USA). SC79 was purchased from Tocris Bioscience (Bristol, United Kingdom). Primary antibodies utilized for the cell signaling technology were as follows: GAPDH (#2118s), H3# (9717), H4 (#2592), AcH3 (#8173), AcH4 (#2591), poly ADP-ribose polymerase(PARP) (#9542), cleaved PARP (#9541), caspase-3 (#9662), cleaved caspase-3 (#9664), E-cadherin (#5296), MMP-9 (#13667), phoshpo-Wee1 (#4910), Myt1 (#4282), Akt (9272), phospho-Akt (p-Akt)(#9271), and anti-rabbit IgG (HRP-linked) (#7074) were purchased from Cell Signaling Technology (Denver, MA, USA). FOXM1 (sc-502) and Cdc2 (sc-163) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, CA, USA).
5
Gastric Cancer Cell Line Cultivation
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The gastric cancer cell lines AGS, SNU-216, NCI-N87, SNU-620, SNU-638, SNU-668, NUGC-3, and MKN-74 were purchased from the Korean Cell Line Bank (Cancer Research Center, Seoul National University, Seoul, Korea) and grown in RPMI1640 medium (Gibco) supplemented with 5% fetal bovine serum (Gibco) and 100 μg/ml antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin, Gibco). Cells (1 × 105 cells/well) were plated in 24-well cell culture plates and grown at 37°C in a humidified, 5% CO2/air atmosphere.
6
Cell Lines for Cancer Research
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Human gastric cancer cell lines PAMC82 and AZ521 were obtained from Beijing Tumor Hospital (Beijing, China) and KCLB (Korean Cell Line Bank, Seoul, Korea), respectively. Human breast cancer cell line T47D was obtained from ATCC (American Type Culture Collection, Rochville, MD, USA). SNU-638 cells were obtained from Korean Cell Line Bank. T47D cell line was cultured in Dulbecco's Modified Eagle's Medium (Invitrogen, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Invitrogen), 2 mmol/l L-Glutamine (L-Glu, Fisher Scientific, Ottama, ON, Canada). PAMC82, SNU638 and AZ521 cells were maintained in RPMI 1640 (Invitrogen) supplemented with 10% fetal bovine serum and 2 mmol/l L-Glutamine. All cells were maintained in a humidified incubator with 5% CO2 at 37 °C. Antibodies used in this study were obtained from the following sources: TNIK (GeneTex, Irvine, CA, USA, Cat. GTX13141), phospho-Akt (S473) (Dako, Produktionsvej, Glostrup, Denmark Cat.M3628), phospho-Erk1/2 (Thr202/Tyr204) (Cell Signaling Technologies, Danvers, MA, USA, Cat. 4370), AKT (Cell Signaling Technologies, Cat. 9272), LC3 (Novus Biologics, Littleton, CO, USA, Cat.NB100-2220), and GAPDH (glyceraldehyde 3-phosphate dehydrogenase; Cell Signaling Technologies, Cat. 2118).
7
Comprehensive Cancer Cell Culture Protocol
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Prostate cancer (DU145 (HTB-81)), head and neck squamous cell carcinoma (Fadu (HTB-43)), pancreatic cancer (BxPC3 (CRL-1687)), melanoma (A375M (CRL-3222), MDA-MB-435 (HTB-131), Sk-mel 24 (HTB-71), Sk-mel 28 (HTB-72), WM115 (CRL-1675)), cells were purchased from American Type Culture Collection (Rockville, MD, USA). Ovarian cancer (OVCAR-8) were from the National Cancer Institute/National Institutes of Health (NCI/NIH; Frederick, MD, USA). Gastric cancer cells (SNU638) were purchased from the Korean Cell Line Bank (KCLB). All cell lines were cultured less than 3 months after resuscitation. The cells were cultured according to manufacturers’ instructions, using EMEM (Eagle’s Minimum Essential Medium) from Thermo Fisher Scientific, Inc., (Waltham, MA, USA) for DU145, Fadu, Sk-mel 28, Sk-mel 24 and WM115 cells, DMEM (Dulbecco’s Modified Eagle’s Medium) from Thermo Fisher Scientific, Inc. for A375M, and RPMI-1640 medium (Thermo Fisher Scientific, Inc.) for MDA-MB-435, OVCAR-8 and SNU638 cells, supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA), l -glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin (Sigma-Aldrich Corporation, St. Louis, MO, USA), and incubated at 37 °C in humidified air with 5% CO2.
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