Primescript and rt reagent kit with gdna eraser

The total RNA was extracted for Chinese cabbage tissues using TRIzol, while yeast RNA was extracted using the M5 EASYspin yeast RNA rapid extraction kit, MF158-01 (Mei5 Biotechnology, Co., Ltd.). For yeast RNA extraction, the cells were grown until the OD₆₀₀ value reached 0.3 at 28 °C and then treated with Hg for 18 h before the total RNA was harvested [4 (link)]. The first-stand cDNA was synthesized using a PrimeScript and RT reagent kit with gDNA Eraser (TAKARA). The SYBR Premix Ex-Taq Kit (TAKARA) was used for quantitative real-time PCR. All experiments were performed with three independent biological replications. The transcript levels were calculated using the 2^ΔΔ-CT method. The TMP values of Chinese cabbage tissues were obtained from the Chinese cabbage database (http://brassicadb.cn/#/, accessed on 18 August 2022) for BrCYP71A15 gene. The primers used for qRT-PCR are presented in Supplementary Table S1.

The total RNA was extracted for Chinese cabbage tissues using TRIzol, while yeast RNA was extracted using the M5 EASYspin yeast RNA rapid extraction kit, MF158-01 (Mei5 Biotechnology, Co., Ltd. Beijing China). For yeast RNA extraction, the cells were grown until the OD₆₀₀ value reached 0.3 at 28 °C, and then treated with 1 M NaCl for 12 h before the total RNA was harvested [45 (link)]. The first-stand cDNA was synthesized using a PrimeScript and RT reagent kit with gDNA Eraser (TAKARA). The SYBR Premix Ex-Taq Kit (TAKARA) was used for quantitative real-time PCR. All experiments were performed with three independent biological replications. The transcript levels were calculated using the 2^∆∆-CT method. The TMP values of Chinese cabbage tissues were obtained from the Chinese cabbage database (http://brassicadb.cn (accessed on 22 March 2022)) for each S1fa gene. The primers used for qRT-PCR are presented in Supplementary Table S1.