Ab65344

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab65344 is a lab equipment product manufactured by Abcam. It is a general-purpose laboratory instrument used for various scientific applications. The core function of this product is to perform specific tasks required in a laboratory setting. No further details about its intended use or other interpretations are provided.

Automatically generated - may contain errors

Lab products found in correlation

Ab83362, by Abcam (1 mentions) Ab119597, by Abcam (1 mentions) Unistat, by Leica (1 mentions) Ab102526, by Abcam (1 mentions) Ab216165, by Abcam (1 mentions) Ab65340, by Abcam (1 mentions) Ab197742, by Abcam (1 mentions) Ab102522, by Abcam (1 mentions) Bs70000, by Bioworld Technology (1 mentions) Bs1491, by Bioworld Technology (1 mentions) Ap0063, by Bioworld Technology (1 mentions) Bs1098, by Bioworld Technology (1 mentions) Primescript rt master mix, by Takara Bio (1 mentions) Faststart universal sybr green master mix, by Roche (1 mentions)

5 protocols using ab65344

Alpinia oxyphylla Extract Reduces Uric Acid

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Example 2

Each of 200 mg/kg extract of Alpinia oxyphylla and 50 mg/kg allopurinol as a positive control was suspended in 0.01 M PBS (phosphate buffered saline) containing 0.1% polyoxyethylene sorbitan monooleate, and orally administered once to a SD-rat, which is a normal animal model.

Two hours after the oral administration, the animal was anesthetized with ethyl ether, and blood was taken from the animal. By using a uric acid assay kit (AB65344, ABCAM, USA), the uric acid amount was measured.

As it is illustrated in FIG. 1, the result of measuring uric acid in blood was shown to be about 2.1 mg/dl, as a result of having a decreased uric acid amount according to an administration of the extract of Alpinia oxyphylla. It was confirmed that the uric acid in blood was reduced by 26% compared to the normal group.

US10709754B2. Composition for preventing, ameliorating, or treating hyperuricemia or metabolic disorders related with hyperuricemia comprising extract of Alpinia oxyphylla as effective ingredient (2020-07-14). KOREA INSTITUTE OF ORIENTAL MEDICINE [KR]. Inventors: Dong Seon Kim [KR], Ohn Soon Kim [KR], Yun Mi Lee [KR], Young-Sil Lee [KR], Eun Jung Son [KR].

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Xanthine Oxidase Inhibition Assay

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XO catalyzes the conversion of hypoxanthine to xanthine and then uric acid as a final product in the presence of molecular oxygen to yield superoxide anion [20 (link)]. Inhibitory effects on xanthine oxidase activity were measured by a decrease in uric acid formation. Urine and serum level of uric acid were determined by the uric acid detection kit (ab65344, abcam). The experimental process performed according to manufacturer’s instructions.

Lee S.H., Lee H.S., Park G., Oh S.M, & Oh D.S. (2019). Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine. BMC Complementary and Alternative Medicine, 19, 57.

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Renal Function Evaluation via Biomarkers

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Renal function was evaluated via assessing serum levels of creatinine (Cr; ab700460), uric acid (ab65344), blood urea nitrogen (BUN; ab83362), lactate dehydrogenase (LDH; ab102526), and kidney injury molecule-1 (Kim-1; ab119597) using the specified colorimetric or ELISA kit obtained from Abcam Inc. (Waltham, MA, USA), agreeing with the manufacturer’s practices consuming a spectrophotometer (LEICA UNISTAT^®; Leica Inc., Allendale, NJ, USA).

Mohamed M.E., Kandeel M., Abd El-Lateef H.M., El-Beltagi H.S, & Younis N.S. (2022). The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NFκB Pathway. Antioxidants, 11(3), 535.

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Measuring Serum Uric Acid Levels

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The serum level of uric acid was determined using a uric acid assay kit (ab65344; Abcam, Cambridge, UK) in accordance with the manufacturer’s instructions.

Sato K., Yamazaki K., Kato T., Nakanishi Y., Tsuzuno T., Yokoji-Takeuchi M., Yamada-Hara M., Miura N., Okuda S., Ohno H, & Yamazaki K. (2021). Obesity-Related Gut Microbiota Aggravates Alveolar Bone Destruction in Experimental Periodontitis through Elevation of Uric Acid. mBio, 12(3), e00771-21.

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Febuxostat Attenuates Renal Fibrosis

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PO (156124) and febuxostat (FEB) (SML1285) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The primary antibodies used in the present study were purchased from Bioworld Technology (Nanjing, China) and were as follows: rabbit anti-collagen 3A1, rabbit anti-α-SMA, rabbit anti-fibronectin, rabbit anti-vimentin, rabbit anti-E-cadherin, rabbit anti-NLRP3, rabbit anti-ASC, rabbit anti-caspase-1, rabbit anti-IL-1β, rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and rabbit anti-TGFβ1 (BS7028, BS70000, BS90514, BS1491, BS1098, BS90948, BS6205, BS5641, AP0063, BS72410,BS91338). The assay kits for UA (ab65344), creatinine (ab65340), xanthine oxidase (XOD) (ab102522), IL-1β (ab197742), and IL-18 (ab216165) were purchased from Abcam (Cambridge, UK). The PrimeScript™ RT Master mix was obtained from Takara (Takara, Japan) and the FastStart Universal SYBR Green master mix was obtained from Roche (Basel, Switzerland).

Shui G., Cai Z., Wang F., Chen T., Huang X., Cai Y, & Mi X. (2022). Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation. BMC Complementary Medicine and Therapies, 22, 278.

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