Polyclonal anti ambra1

Manufactured by Novus Biologicals

Sourced in United States

Polyclonal anti-AMBRA1 is a laboratory reagent that recognizes the AMBRA1 protein. AMBRA1 is a key regulator of autophagy, a cellular process involved in the degradation and recycling of cellular components. This antibody can be used to detect and study the expression and localization of AMBRA1 in various biological samples.

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Lab products found in correlation

Monoclonal anti bcl 2, by Santa Cruz Biotechnology (2 mentions) Polyclonal anti lc3, by Cell Signaling Technology (2 mentions) Monoclonal anti gapdh, by Abcam (1 mentions) Mouse monoclonal anti actin, by Merck Group (1 mentions) Mouse monoclonal anti myc 9e10, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti flag, by Merck Group (1 mentions)

2 protocols using polyclonal anti ambra1

Antibody Profiling of Cellular Proteins

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The antibodies used were as follows: polyclonal anti-PARKIN (Santa Cruz Biotechnology, Santa Cruz, CA, USA, H-300- sc-30130, Abcam, Cambridge, UK, 15954), mouse monoclonal anti-ACTIN (Sigma-Aldrich, St. Louis, MO, USA), monoclonal anti-BCL-2 (Santa Cruz Biotechnology, sc-7382), polyclonal anti-MnSOD (Assay Designs, Danvers, MA, USA), polyclonal anti-AMBRA1 (Novus Biologicals, Littleton, CO, USA, 26190002), polyclonal anti-LC3 (Cell Signaling, Danvers, MA, USA), monoclonal anti-GAPDH and rabbit polyclonal anti-LAMP1 (Abcam 24170).

Strappazzon F., Nazio F., Corrado M., Cianfanelli V., Romagnoli A., Fimia G.M., Campello S., Nardacci R., Piacentini M., Campanella M, & Cecconi F. (2014). AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1. Cell Death and Differentiation, 22(3), 419-432.

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Comprehensive Immunoblotting Antibody Panel

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Mouse monoclonal anti-ACTB/β actin (Sigma-Aldrich, A2228), monoclonal anti-BCL2 (Santa Cruz Biotechnology, sc-7382), polyclonal anti-AMBRA1 (Novus, 26190002), monoclonal anti-AMBRA1 (Santa Cruz Biotechnology, sc-398204), mouse monoclonal anti-CYCS (Assay designs-Enzo Life Sciences, 6H2-B4), polyclonal anti-LC3 (Cell Signaling Technology, 2775), monoclonal anti-SQSTM1/p62 (Santa Cruz Biotechnology, sc-28369), polyclonal anti-activated BAX (6A7; Abcam 5714), polyclonal anti-BAX (Santa Cruz Biotechnology, sc-493, N-20), polyclonal anti-PARP1 (Cell Signaling Technology, 9542), goat anti-BAK1 (G-23; Santa Cruz Biotechnology, sc-832), mouse monoclonal anti-MYC (9E10; Santa Cruz Biotechnology, sc-4), mouse monoclonal anti-Flag (Sigma-Aldrich, F3165), rabbit polyclonal anti-SOD2/MnSOD (Enzo Life Sciences, 110F) and mouse monoclonal anti-TUBB/β tubulin (Sigma-Aldrich, T9026).

Strappazzon F., Di Rita A., Cianfanelli V., D'Orazio M., Nazio F., Fimia G.M, & Cecconi F. (2016). Prosurvival AMBRA1 turns into a proapoptotic BH3-like protein during mitochondrial apoptosis. Autophagy, 12(6), 963-975.

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