The following antibodies and concentrations were used: rabbit anti-Gγ13 (1:500, a gift from R. Margolskee); rabbit anti-Gβ3 (1:300, HPA005645; Sigma); guinea pig anti-mGluR6 (1:100, Neuromics Edina, MN Oregon); mouse anti-Gαo (1:100, MAB 3073; Millipore); rabbit anti-TRPM1 (1:100, a gift from T. Furukawa, Osaka Bioscience Institute, Osaka, Japan) (Koike et al., 2010 (link); Kondo et al., 2011 (link)); rabbit anti-Gβ5 (1:500) and rabbit anti-RGS11 (1:1000, gifts from T. Wensel, Baylor College of Medicine, Houston, TX); rabbit anti-RGS7 (1:200, gift from K. Martemyanov, Scripps Research Institute, Jupiter, FL); rabbit anti-Ret-PCP2 (1:1,000, gift of Dr. Rod Feddersen; see Xu et al. 2008 (link)); rabbit anti-pikachurin (1:2,000, Wako 011-22631, Richmond,VA); mouse anti-dystroglycan (1:100, Abcam, ab49515, Cambridge, MA); mouse anti-dystrophin (1:100, Millipore MAB 1694, Billerica, MA); rabbit anti-RGS9 anchoring protein (R9AP) (1:100–500, a gift from V. Arshavsky, Duke University, Durham, NC); mouse anti-kinesin [1:100–200, Covance (currently Bio-legends)]; rabbit anti-ribeye (1:500, a gift from T. Sudhof, Stanford Universty, CA); sheep anti-TRPM1 (for Western blotting,1:1,000, Gift of Dr. Kirill Martemayanov, The Scripps Research Institute, Jupiter, Florida); and mouse anti-beta-actin (1:3,000, Clone AC-74 Sigma, St. Louis, MO).
Mab3073
Manufactured by Merck Group
Sourced in Japan, United States
MAB3073 is a laboratory instrument developed by Merck Group. It is designed for performing advanced analytical techniques in scientific research and industrial applications. The core function of MAB3073 is to enable precise measurements and analysis of various samples and materials.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 880, by Zeiss (2 mentions)
Rabbit anti pikachurin, by Fujifilm (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
S11223, by Thermo Fisher Scientific (1 mentions)
Alexa 594, by Thermo Fisher Scientific (1 mentions)
Ab234, by Evrogen (1 mentions)
Ab144p, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions)
Mab5406, by Merck Group (1 mentions)
010399 clone 6b3, by Swant (1 mentions)
L 21409, by Thermo Fisher Scientific (1 mentions)
Ab15282, by Merck Group (1 mentions)
Sc 10800, by Santa Cruz Biotechnology (1 mentions)
4 protocols using mab3073
1
Retinal Protein Antibody Characterization
2
Retinal immunohistochemistry for optogenetics
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At the end of the terminal experiment, mice were euthanized and retinas extracted for subsequent immunohistochemistry to confirm retinal expression patterns of the optogenes. Immunohistochemistry of cryosections were similar to that described previously26 (link). In brief, retinas or eyecups were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) for 30 min. Antibodies were diluted in a blocking solution containing 1% Triton-X and 2% donkey serum. Sections were incubated overnight at 4 °C in primary antibody and 2 h in secondary antibody at room temperature. The following primary antibodies were used: rabbit anti-tRFP (1:1000; Evrogen; AB234), rabbit anti-melanopsin (1:1000; Advanced Targeting Systems; AB-N39), goat anti-ChAT (1:100; Millipore; AB144P) and mouse anti-Goα (1:1000; Millipore; mab3073). Secondary antibodies were always from donkey and either conjugated to Alexa 488 or Alexa 594 (1:400; Invitrogen). Alexa 488 conjugated to streptavidin was used to visualize cells injected with biocytin during patch-clamp experiments (1:400; Invitrogen; S-11223). Nuclei were stained with 10 μg/ml DAPI (Roche). Micrographs were taken on a Zeiss LSM 880. Processing of image stacks was done using ImageJ (Rasband WS, United States National Institutes of Health, Bethesda, Maryland, US).
3
Immunolabeling of Retinal Cell Types
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Retinal sections and whole mounts were processed with the following primary antibodies (Table 1 ) and dilutions: rabbit polyclonal antibody against Prox1 (1:1000–1:2000, PRB-238C, BioLegend, San Diego, CA, USA), mouse monoclonal antibody against calbindin (1:1000, C9848, cl. CB-955; Sigma-Aldrich, St. Louis, MO, USA), mouse polyclonal antibody against calretinin (1:5000, 010399 clone 6B3; Swant, Bellinzona, Switzerland), mouse monoclonal antibody to glutamic acid decarboxylase 67 (GAD67; 1:1000, MAB5406; Millipore, Temecula, CA, USA), mouse monoclonal antibody to Goα (1:300, MAB3073; Millipore, Temecula, CA, USA) and rat polyclonal antibody against glycine (1:1000; IG1002; ImmunoSolution, Everton Park, QLD, Australia). Prox1 antiserum was generated against the C-terminal 15 amino acids of mouse Prox1 (manufacturer’s technical information).
4
Retinal Neurotransmitter Receptor Localization
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