Deparaffinized and hydrated of paraffin-embedded lung sections. The expression of haemagglutinin (HA) and the activation of STAT pathway were assessed using anti-HA (1:2000; Sino Biological), anti-pSTAT1 (Tyr701) antibody (1:200, Cell Signalling Technology, MA, USA) and anti-pSTAT3 (Tyr705) antibody (1:200, Cell Signalling Technology). Macrophage infiltration was explored using anti-F4/80 antibody (1:200, Cell Signalling Technology). Neutrophil recruitment was assessed using anti-Ly6G antibody (1:200, Cell Signalling Technology). The antibody was detected by streptavidin-biotin (Beijing Zhongshan Biotechnology Co., Ltd., Beijing, China). Five slides were randomly selected from the whole slides, and then evaluated using Image J pro.
Anti ly6g antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-Ly6G antibody is a laboratory research tool used to detect the presence and distribution of the Ly6G protein in biological samples. Ly6G is a cell surface marker expressed on mature neutrophils, a type of white blood cell. The antibody can be used in various experimental techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and study neutrophils in different contexts.
Automatically generated - may contain errors
Lab products found in correlation
Anti p stat3 tyr 705 antibody, by Cell Signaling Technology (1 mentions)
Anti ha, by Sino Biological (1 mentions)
Anti f4 80 antibody, by Cell Signaling Technology (1 mentions)
Anti lipocalin 2 ngal, by Abcam (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Nanozoomer s360, by Hamamatsu Photonics (1 mentions)
3 protocols using anti ly6g antibody
1
Immunohistochemical Analysis of Influenza Infection
2
Quantitative Immunohistochemistry for Ly6G and NGAL
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The anti-Ly6G antibody (1:200, Cell Signaling), anti-Lipocalin-2/NGAL (1:1000, abcam) were used for IHC. Images were taken using Nikon ECLIPSE Ni-U. Image J software (NIH) was used for process and quantify Ly6G-positive areas in the images.
3
Immunohistochemical Staining of Ly6G
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After fixation, tissues were paraffin-embedded and cut into 4 μm sections. The sections then underwent xylene deparaffinization, rehydration with 30–100% ethanol, and boiling in retrieval buffer (10 mM citrate buffer, pH 6.0) for 5 min. Tissue sections were blocked with 5% bovine serum albumin and incubated with an anti-Ly6G antibody (1:100) (Cell Signaling Technology, Boston, MA, USA) at 4 °C overnight. Afterward, the sections were then incubated with a secondary antibody (1:200) at room temperature for 30 min. After staining with 3,3'-diaminobenzidine (Sigma-Aldrich, Saint Louis, MO, USA) and counterstaining with hematoxylin and eosin (H&E) (Yeasen, Shanghai, China), the tissue sections were scanned with NanoZoomer S360 (Hamamatsu Photonics, Naka-ku Hamamatsu City, Shizuoka Pref., Japan).
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