A Bruker UltraFlex
L matrix-assisted laser desorption ionization time-of-flight mass
spectrometry (MALDI-TOF MS) system with a
SCOUT-MTP ion source (Bruker
Daltonics, Bremen) was used. All spectra were acquired using a reflector-positive
mode. The instrument was calibrated by using SpheriCal TM calibrants
obtained from Polymer Factory Sweden AB. Matrixes were prepared by
the dissolution of the matrix substance at a concentration of 10 mg/mL,
salt at a concentration of 1 mg/mL, and analyte at a concentration
of 1 mg/mL in THF. The samples were prepared at a ratio of 20:5:5
of the matrix substance, monomer analyte, and counter ion, respectively.
The matrix substance 2,5-dihydroxybenzoic acid (DHB) and the counterion
salt sodium trifluoroacetate (NaTFA) were used for mass analysis unless
otherwise stated. The received spectra were analyzed with FlexAnalysis
Bruker Daltonics, Bremen, version 2.2.
Lin J., Fan Y., Hutchinson D.J, & Malkoch M. (2023). Soft Hydroxyapatite Composites Based on Triazine–Trione Systems as Potential Biomedical Engineering Frameworks. ACS Applied Materials & Interfaces, 15(5), 7329-7339.