Microfluidics experiments were conducted with the CellASIC® ONIX2 microfluidic system (EMDMillipore, Burlington, MA) following manufacturer’s protocols. Briefly, 10,000 cells were loaded into each chamber of a CellASIC ONIX M04 switching plate and allowed to incubate overnight in complete media. The next day, stock solutions of 110nM AZD5153 and 58nM AZD5153, DMSO controls and additional complete media were loaded into the microfluidic plate, which was then placed inside a XLMulti S1 incubator (Pecon, Erbach, Germany) mounted onto a LSM800 confocal inverted laser scanning microscope (Zeiss, Oberkochen, Germany). Cells were kept at 37°C and supplemented with 5% CO2. Images were captured every 30 minutes over a 72-hour period. Drug dosing schedule replicated human area under the curve (AUC) clinical serum concentrations (G. Pouliot, personal communication, November 19, 2019). Cell surface area was quantified using FIJI ImageJ software.
Xlmulti s1 incubator
Manufactured by Pecon
Sourced in Germany
The XLMulti S1 incubator is a laboratory equipment designed for incubation purposes. It provides a controlled environment for various applications that require temperature regulation and monitoring.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using xlmulti s1 incubator
1
Microfluidic Analysis of AZD5153 Effects
2
Microfluidic Imaging of AZD5153 Responses
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Microfluidics experiments were conducted with the CellASIC® ONIX2 microfluidic system (EMDMillipore, Burlington, MA) following manufacturer's protocols. Briefly, 10,000 cells were loaded into each chamber of a CellASIC ONIX M04 switching plate and allowed to incubate overnight in complete media. The next day, stock solutions of 110nM AZD5153 and 58nM AZD5153, DMSO controls and additional complete media were loaded into the microfluidic plate, which was then placed inside a XLMulti S1 incubator (Pecon, Erbach, Germany) mounted onto a LSM800 confocal inverted laser scanning microscope (Zeiss, Oberkochen, Germany). Cells were kept at 37˚C and supplemented with 5% CO2.
Images were captured every 30 minutes over a 72-hour period with Zen2.5 software. Drug dosing schedule replicated human AUC concentrations as published (AstraZeneca data). Cell surface area was quantified using FIJI ImageJ software.
Images were captured every 30 minutes over a 72-hour period with Zen2.5 software. Drug dosing schedule replicated human AUC concentrations as published (AstraZeneca data). Cell surface area was quantified using FIJI ImageJ software.
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