Total protein sample from cell lines was extracted with RIPA lysis buffer (Sigma-Aldrich, USA) and corresponding protein concentration was determined with a BCA protein assay kit (Beyotime). Equal amount of protein sample was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Millipore). After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against ZFP36L2, E-cadherin, N-cadherin, Vimentin and GAPDH (Abcam, Cambridge, MA, USA) overnight at 4 °C. Following incubation with corresponding secondary antibodies for 2 h at room temperature, the protein bands were visualized with Chemiluminescence Substrate Kit (Thermo Fisher Scientific).
Zfp36l2
Manufactured by Abcam
Sourced in United States
ZFP36L2 is a protein that functions as a zinc finger protein. It is involved in the regulation of mRNA stability and translation.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence substrate kit, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
E cadherin, by Abcam (1 mentions)
N cadherin, by Abcam (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Ubiquitin, by Cell Signaling Technology (1 mentions)
P h2ax, by Cell Signaling Technology (1 mentions)
Redd1, by Proteintech (1 mentions)
Novex bis tris poly acrylamide gel, by Thermo Fisher Scientific (1 mentions)
Sesn2, by Proteintech (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using zfp36l2
1
Protein Expression Analysis of Cell Lines
2
Protein Expression Analysis by Western Blot
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Proteins were resolved on 4%–12% Novex Bis-Tris poly-acrylamide gel (Invitrogen) and blotted onto nitrocellulose membrane (Invitrogen). Membranes were incubated with the following primary antibodies overnight, before the addition of HRP-conjugated secondary antibodies. P-P70S6K (T389), P70S6K, P-S6 (S240/244), S6, P-AKT (S473), AKT, P-AMPK (T172), AMPK, TSC2, P53 (1C12), Ubiquitin, P-H2AX, and GAPDH were from Cell Signaling Technology. HPRT-1, REDD1, SESN2, and MDM2 were from Proteintech. ZFP36L2 was obtained from Abcam, MilliporeSigma, and Santa Cruz Biotechnology. The presence of target protein was visualized using Super Surgical Western Pico ECL substrate (Pierce). Quantification of Western blotting images was done using ImageJ (NIH). Antibody suppliers and catalog numbers are shown in Supplemental Table 4 .
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