Fluorescein isothiocyanate fitc conjugated anti cd44

Manufactured by BD

Fluorescein isothiocyanate (FITC)-conjugated anti-CD44 is a laboratory reagent used for the detection and analysis of CD44 expression on cells. FITC is a fluorescent dye that is covalently attached to the anti-CD44 antibody, allowing for the visualization and quantification of CD44-positive cells through flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Pe conjugated anti cd24, by BD (1 mentions) Guava easycyte flow cytometer, by Merck Group (1 mentions) Facsdiva software, by BD (1 mentions)

2 protocols using fluorescein isothiocyanate fitc conjugated anti cd44

Breast Cancer Stem Cell Profiling

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By using a Guava easyCyte flow cytometer (Millipore), the expression of stem cell markers in a breast cancer panel was distinctly evaluated in cells from mammospheres. The antibodies used were phycoerythrin (PE)-conjugated anti-CD24 (BDbiosciences) and fluorescein isothiocyanate (FITC)-conjugated anti-CD44 (BDbiosciences). Staining was done according to the instructions of the manufacturer and analysed in flow cytometer. For the analysis, 80,000 cells were detected and quantified by percentage (%).

Gonçalves N.D., Colombo J., Lopes J.R., Gelaleti G.B., Moschetta M.G., Sonehara N.M., Hellmén E., Zanon C.D., Oliani S.M, & Zuccari D.A. (2016). Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines. PLoS ONE, 11(3), e0150407.

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Quantifying Breast Cancer Stem Cells

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The expression of breast cancer stem cell markers was distinctly measured in cells with different treatment. PE (phycoerythrin )-conjugated anti-CD24 (BDbiosciences) and fluorescein isothiocyanate (FITC)-conjugated anti-CD44 antibodies were purchased from BDbiosciences. Staining was performed following the manufacturer′s instructions and analyzed with flow cytometer (BD Biosciences) using FACSDiVa software (BD Biosciences) in which 20000 cells were examined and quantified by percentage (%).

Zhang L., Wang H., Li C., Zhao Y., Wu L., Du X, & Han Z. (2017). VEGF-A/Neuropilin 1 Pathway Confers Cancer Stemness via Activating Wnt/β-Catenin Axis in Breast Cancer Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(3).

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