Invivoscope 2

The mice carrying LNCaP xenografts (n = 9) or DU 145 xenografts (n = 4) were injected intravenously (i.v.), through tail-vein injections, with ¹⁷⁷Lu-m11B6 (7.9 ± 0.69 MBq, 30 μg mAb, in approximately 100 μL PBS) for small-animal SPECT/CT (Bioscan) imaging, using the NSP-106 multi-pinhole mouse collimator. Energy windows of 20 % were centered over the 113 and 208 keV energy peaks [20 ]. SPECT data were reconstructed using HiSPECT software (SciVis, Goettingen, Germany). Three mice belonging to the LNCaP xenograft group had been pre-dosed 24 h prior to injection with 1 mg of unlabeled m11B6. These animals were imaged at 72 h or 168 h p.i. and euthanized and dissected as follows: 72 h (LNCaP, n = 3; pre-dosed, n = 3; and DU 145: n = 4) or 168 h (LNCaP, n = 3). The organs and tissues were measured for activity content in an automated well counter with a 3-in. NaI(Tl) detector (1480 WIZARD, Perkin Elmer), as described below. SPECT data analysis and quantification were done in InVivoScope 2.0 software (inviCRO, Boston, MA, USA), and regions of interest (ROIs) were drawn using the CT image as an anatomical reference.

5 days following treatment injection, 3 animals (2 with specific RIT and one with non specific RIT) were imaged using a small animal SPECT Imaging camera (NanoSPECT-CT Plus; BIOSCAN, Washington, DC, USA) and whole body SPECT/CT tomographic images were acquired with multi pinhole collimators. Briefly, mice were place under gaseous anesthesia and SPECT/CT images were acquired sequentially using Nucline software (Mediso, Hungary). Co-registration of images was carried out using InVivoScope 2.0 software (inviCRO, USA). Quantification analyses were performed with VivoQuant 2.5 software (inviCRO, USA).