For screening, a qPCR-based array was performed as previously described [16 (link)]. Briefly, total RNA was isolated from serum samples of the 20 active BP patients and 20 healthy controls with a miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany). The yield of total RNA was 7-15 ng/μL. The cDNA was obtained from 3 μL of RNA with use of the TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems, Beverly, USA). The TaqMan® PreAmp Kit (Applied Biosystems) was used for preamplifying with 2.5 μL of cDNA product per specimen. The qPCR-based array analysis was performed with use of the TaqMan Low Density Array Human MicroRNA Panel (CapitalBio, Beijing, China). A total of 768 known miRNAs were quantified with use of the TaqMan® Human MicroRNA Array A and B (Applied Biosystems). Raw cycle threshold (Ct) values were calculated by SDS 2.4 and RQ manager 1.2 software (Applied Biosystems). A Ct value of ≥35.0 was used as a cut-off.
Taqman low density array human microrna panel
Manufactured by CapitalBio
Sourced in China
The TaqMan Low Density Array Human MicroRNA Panel is a laboratory equipment product. It is designed for the analysis of human microRNA expression. The panel allows for the simultaneous quantification of multiple microRNA targets using real-time PCR technology.
Automatically generated - may contain errors
Lab products found in correlation
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Taqman preamp kit, by Thermo Fisher Scientific (2 mentions)
Mirneasy serum plasma kit, by Qiagen (2 mentions)
Rq manager 1, by Thermo Fisher Scientific (2 mentions)
Taqman human microrna array a and b, by Thermo Fisher Scientific (1 mentions)
Sds 2, by Thermo Fisher Scientific (1 mentions)
2 protocols using taqman low density array human microrna panel
1
Serum miRNA Profiling in Bullous Pemphigoid
2
Profiling Circulating microRNAs in Chronic Spontaneous Urticaria
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Total RNA was isolated from serum samples (200 μl per sample) of 20 CSU patients and 20 controls using a miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany) according the manufacturer's protocols. In general, the yield of total RNA was 2-10 ng/μl. The cDNA was obtained from 3 μl of RNA using the TaqMan ® microRNA Reverse Transcription Kit (Applied Biosystems, Beverly, MA, USA). The cDNA product per specimen (2.5 μl) was then pre-amplified using the TaqMan ® PreAmp Kit (Applied Biosystems). Quantitative PCR (qPCR)-based array analysis was performed using the TaqMan Low Density Array Human MicroRNA Panel (Capitalbio, Beijing, China). A total of 768 known human miRNAs were quantified using the TaqMan ® Human MicroRNA Array A and B kit (Applied Biosystems). Raw cycle threshold (Ct) values were calculated using SDS 2.4 and RQ manager 1.2 software (Applied Biosystems) and applied automatic baseline and threshold settings. A Ct value greater than or equal to 35.0 was discarded.
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