The NMIC/ID-4 panel of the BD Phoenix system was employed to analyze a total of 1022 bacterial isolates. Initially, a McFarland 0.5 stock solution was prepared in 4.5 mL of Phoenix ID broth [17 (link)] using a Sensi Titre™ nephelometer (Thermo Fisher Scientific, Waltham, MA, USA). The resulting bacterial ID solution was then placed in the designated ID field on the Phoenix panel. Subsequently, 50 µL of the microbial solution was added to each chemical reactivity well on the panel. Any excess suspension was absorbed by the cushion located on the underside of the panels. The panel was sealed with a rubberized coating, and the display code was read before being directly inserted into the Phoenix machine.
For the cultivated bacterial isolates, the ID results obtained from the Phoenix system were compared with those of the traditional API system, which served as the reference standard. Accurate identification rates were subsequently determined at both the genus and species levels based on these comparisons.
For the cultivated bacterial isolates, the ID results obtained from the Phoenix system were compared with those of the traditional API system, which served as the reference standard. Accurate identification rates were subsequently determined at both the genus and species levels based on these comparisons.