Cyclin e1

Manufactured by BD

Cyclin E1 is a protein that plays a key role in the regulation of the cell cycle. It is involved in the transition from the G1 phase to the S phase of the cell cycle, which is the phase where DNA replication occurs. Cyclin E1 binds to and activates the cyclin-dependent kinase 2 (CDK2) enzyme, which is essential for the initiation and progression of DNA replication.

Automatically generated - may contain errors

Lab products found in correlation

Bcl 2, by Agilent Technologies (1 mentions) Bcl2 associated x (bax), by R&D Systems (1 mentions) Phosphor stat3, by Cell Signaling Technology (1 mentions) Cyclin a1, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Active β catenin, by Merck Group (1 mentions) E cadherin, by BD (1 mentions) Iwr 1 endo, by Santa Cruz Biotechnology (1 mentions) Phospho and total akt, by Cell Signaling Technology (1 mentions) Ly294002, by Merck Group (1 mentions) Total β catenin, by BD (1 mentions) Para nitrophenyl phosphate, by Merck Group (1 mentions) Recombinant human ccl25, by R&D Systems (1 mentions) Gemcitabine, by LC Laboratories (1 mentions) Wnt3a, by R&D Systems (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using cyclin e1

Protein Quantification and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples used for this assay were collected from whole-cell lysates. A coomassie assay (Pierce, Rockford, IL) was used to quantify the total protein concentration. Identical amounts of protein were run on sodium dodecyl sulfate polyacrylamide gel electrophoresis gels and electro-transferred to polyvinylidene fluoride membranes. The following primary antibodies were used in this study: Bim, Noxa, PUMA (ProSci, Poway, CA); Mcl-1, caspase 3, and caspase 8 (BD Biosciences); Bcl-2 (DAKO, Carpinteria, CA); cyclin E1 (BD Biosciences), Bax (R&D Systems), Actin (Santa Cruz Biotechnology), Bcl-xL, Bad, cyclin D1, cyclin A1, STAT3, and phosphor-STAT3 (Cell Signaling Technology).

Xu J., Huang F., Yao Z., Jia C., Xiong Z., Liang H., Lin N, & Deng M. (2019). Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression. Cell Communication and Signaling : CCS, 17, 85.

+ Open protocol

+ Expand

Characterization of CCL25 Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human CCL25 was obtained from R&D Systems (Minneapolis, MN). Additional reagents include: 10 mM paranitrophenyl phosphate (Sigma Aldrich; St. Louis, MO), gemcitabine (LC Laboratories; Woburn, MA), WNT3a (R&D Systems), IWR-1-endo, a WNT inhibitor (Santa Cruz Biotechnology; Santa Cruz, CA), 1-[1,1′-biphenyl]-4-yl-2-(2,3-dihydro-9H-imidazo[ 1,2-a]benzimidazol-9-yl) ethanone hydrobromide (CCT), a β-catenin inhibitor (R&D Systems), and LY294002, a phosphoinositol-3 kinase (PI3K) inhibitor (Sigma Aldrich). Treatment with CCL25 was performed at a concentration of 400 ng/ml. The following antibodies were used: active β-catenin (unphosphorylated on serine 37) (Millipore; Billerica, MA), total β-catenin (BD Bioscience; Sparks, MD), phospho and total AKT (Cell Signaling; Beverly, MA), Cyclin E1 (BD Bioscience), Cyclin D1 (Santa Cruz Biotechnology), E-Cadherin (BD Bioscience), p84 (Genetex; Irvine, CA), and GAPDH (Advanced Immunochemical; Long Beach, CA).

Lee S., Heinrich E.L., Li L., Lu J., Choi A.H., Levy R.A., Wagner J.E., Yip M.L., Vaidehi N, & Kim J. (2015). CCR9‐mediated signaling through β‐catenin and identification of a novel CCR9 antagonist. Molecular Oncology, 9(8), 1599-1611.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!