Dkk100

Manufactured by R&D Systems

Sourced in United States

The DKK100 is a laboratory instrument designed for measuring dissolved oxygen concentrations. It features a digital display and provides precise oxygen level readings. The core function of the DKK100 is to quantify the amount of dissolved oxygen in liquid samples.

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Lab products found in correlation

High performance liquid chromatography system, by Bio-Rad (1 mentions) Cobas 8000, by Roche (1 mentions) Au2700, by Olympus (1 mentions) Multiskan mk3, by Thermo Fisher Scientific (1 mentions)

2 protocols using dkk100

Metabolic Indices and Renal Function

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Medical history and clinical characteristics (sex, age, height, weight, and blood pressure) were recorded in the morning during patients’ clinic visits. Blood samples for analysis of metabolic indices were also collected. Urine samples were obtained for urinary albumin and creatinine testing. Body mass index (BMI), UACR, and estimated glomerular filtration rate (eGFR) were calculated. HOMA-IR [fasting glucose × fasting insulin (μU/mL)/22.5] was used to calculate insulin resistance.
Renal function and lipids were assessed using an autoanalyzer (Cobas 8000, Roche, Basel, Switzerland). Hemoglobin A1c (HbA1c) was detected by using a high-performance liquid chromatography system (Bio-Rad, United States). Fasting insulin and C-peptide levels were tested by chemiluminescence (e601, Roche). Serum Dickkopf-1 concentrations were assayed by ELISA employing a human Dickkopf-1-specific antibody, with a range of 10 to 1000 pg/mL (R&D systems Catalog DKK100, United States). Urinary albumin was measured by immune turbidimetry and creatinine was measured with a chemistry analyzer (AU2700, Olympus, Tokyo, Japan).

Hou N.N., Kan C.X., Huang N., Liu Y.P., Mao E.W., Ma Y.T., Han F., Sun H.X, & Sun X.D. (2021). Relationship between serum Dickkopf-1 and albuminuria in patients with type 2 diabetes. World Journal of Diabetes, 12(1), 47-55.

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Quantifying Serum DKK-1 Protein by ELISA

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Serum levels of DKK‐1 protein were measured by enzyme‐linked immunosorbent assay (ELISA) with a commercially available kit (R&D Systems, Minneapolis, MN, Catalog No. DKK100). Briefly, the concentrations of the DKK‐1 standards for creating a standard curve were 0, 31.2, 62.5, 125, 250, 500, 1000, and 2000 pg/mL. A total quantity of 100 μL of Standard and sample (an eightfold dilution) were added and incubated for 2 h at room temperature, followed by the addition of 200 μL of the Dkk‐1 Conjugate (a polyclonal antibody against Dkk‐1 conjugated to horseradish peroxidase [HRP]) for another 2 h at room temperature. Color development was achieved with 200 μL substrate solution per well for 30 min, and sulfuric acid as stop solution was added. The optical density was read at 450 nm and referenced to 630 nm on a plate microplate reader (Multiskan MK3; Thermo Fisher Scientific). All measurements were run in duplicate. The concentrations of DKK‐1 were obtained with a standard curve, fitted for the standard value, and multiplied by the dilution factor.

Peng Y., Xu Y., Guo H., Huang L., Tan H., Hong C., Li S., Xu L, & Li E. (2016). Combined detection of serum Dickkopf‐1 and its autoantibodies to diagnose esophageal squamous cell carcinoma. Cancer Medicine, 5(7), 1388-1396.

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