For immunohistochemical analysis, the sections of transplanted cardiac grafts were incubated with polyclonal primary antibodies: anti-inducible nitronic oxide synthase (iNOS) (1:100, Abcam, Cambridge, UK) and anti-arginase 1 (Arg-1) (1:100, Cell Signaling Technology, Danvers, MA, USA) respectively. Nonspecific staining was assessed by negative control sections, which omitted the primary antibodies. The quantification was done by ImageJ (version 1.51; National Institutes of Health, USA).
Anti arginase 1 arg 1
Anti-arginase 1 (Arg-1) is a laboratory reagent used in research applications. It is a specific antibody that recognizes and binds to the arginase 1 protein, which is involved in the urea cycle. The primary function of this product is to enable the detection and study of arginase 1 in biological samples.
Lab products found in correlation
4 protocols using anti arginase 1 arg 1
Cardiac Graft Macrophage Characterization
For immunohistochemical analysis, the sections of transplanted cardiac grafts were incubated with polyclonal primary antibodies: anti-inducible nitronic oxide synthase (iNOS) (1:100, Abcam, Cambridge, UK) and anti-arginase 1 (Arg-1) (1:100, Cell Signaling Technology, Danvers, MA, USA) respectively. Nonspecific staining was assessed by negative control sections, which omitted the primary antibodies. The quantification was done by ImageJ (version 1.51; National Institutes of Health, USA).
Evaluating BETi and M2-like Macrophage Markers
Western Blot Analysis of Arginase-1
Microglial Immunophenotyping by Flow Cytometry
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