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3 protocols using curcumin

1

Curcumin-loaded Gal-BSA Nanoparticles

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Gal-BSA-Cur NPs were prepared by our laboratory and sterilized by ultraviolet (UV). Curcumin was purchased from Adamas (Shanghai, China). Sodium carboxyl methyl cellulose (CMC-Na) was purchased from Aladdin (Shanghai, China). Methanol (HPLC grade) was obtained from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). Deionized water was obtained from Watsons. Tetrazolium salt (MTT), dimethyl sulfoxide (DMSO), trypsin solution (0.25%) and sucrose were all provided by Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Lonsa Science Srl Co., Ltd. (Shanghai, China). Dulbecco’s modified eagle’s medium (DMEM) with high glucose and penicillin-streptomycin solution and nonessential amino acids were supplied by Thermo Fisher Scientific Biological Chemical Co., Ltd. (Beijing, China). Heparin was purchased from Sigma (Shanghai, China). Capillary for blood collection was supplied by Taizhou Jiaojiang Glass Instrument Factory. (Taizhou, China). Anticoagulation tube was purchased from Jiangsu Datang Medical Devices Co., Ltd. (Jiangsu, China).
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2

Encapsulation of Curcumin in Cellulose Nanocrystals

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Zein (protein content: 91.3%), pepsin from porcine gastric mucosa (P7000), porcine pancreatic lipase type 2 (L3126) and bile salts (1:1 mixture of cholic acid and deoxycholic acid, 48305) were purchased from Sigma-Aldrich (USA). The pepsin has a reported activity of 474 units/mg and the lipase had a reported activity of 100-500 units/mg (using olive oil). The CNCs with a diameter of 5-20 nm and length of 100-200 nm were obtained from Shanghai ScienceK Nanotechnology Ltd. Cellulose nanocrystals (CNCs) were isolated by sulfuric acid hydrolysis of wood fibers.
Curcumin (greater than 98%) (CAS: 458-37-7) was obtained from Adamas-Beta (Shanghai, China). Other chemical agents and materials were acquired from Eshowbokoo Biological Technology (China).
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3

Synthesis and Characterization of CTPP-Curcumin Nanoparticles

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3-Carboxypropyl-triphenylphosphonium bromide (CTPP) was obtained from Adamas Reagent Co., Ltd (Shanghai, China). Polyethylene glycol (PEG), ε-caprolactone was obtained from Daigang Biomaterial (Shandong, China). N,N′-Dicyclohexylcarbodimide (DCC) and dimethylaminopyridine (DMAP) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Curcumin was purchased from Adamas Reagent Co., Ltd (Shanghai, China). LysoTracker Red, 4% paraformaldehyde and Hoechst 33258 were obtained from Beyotime Biotechnology (Shanghai, China). RPMI-1640 medium and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from KeyGen Biotech (Nanjing, China). Fetal bovine serum (FBS) was purchased from Gibco (Hangzhou, China). All the other chemicals and reagents were analytic grade.
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