Ttl pulse generating box

Behavioral tests with optogenetic stimulation in the PFC were done 2–4 weeks after viral injection. Tests with stimulation in the NAc core were done approximately 6 weeks after injection to ensure optimal expression of opsins. Prior to behavioral tests, optic fibers were connected to a 589 nm laser diode through an FC/PC adapter (Shanghai Dream Lasers, Shanghai), and laser intensity was measured with a power meter (Thorlabs, Newton, NJ, USA). The laser was delivered using a TTL pulse-generating box (Tucker-Davis Technologies, Alachua, FL, USA).

Prior to behavioral tests, and 15 min after bilateral intracranial administrations, optic fibers were connected to a 473 nm laser diode with an FC/PC adapter (Shanghai Dream Lasers, China). And the laser intensity was measured with an instrument of power meter (Thorlabs, Newton, NJ, USA). The output of laser was delivered using a TTL pulse-generating box (Tucker-Davis Technologies, USA), and then was split evenly to two fibers (for bilateral stimulation) with a splitter. Before behavioral tests, laser output in each terminal of the two fibers was verified with an instrument of power meter to ensure that equal power was provided. As previously described [6 (link), 7 (link)], a laser protocol that included alternating light-on and light-off epochs for 30 s each was provided for the duration of Hargreaves test, mechanical allodynia test, cold allodynia test, and CPP test. Light was delivered at 20 Hz with 10 ms pulse length within the light-on epoch.

Animals used for behavior received either AAV1.CAMKII.ChR2-eYFP.WPRE.hGH or AAV1. CAMKII.eYFP.WPRE.hGH (control group) in the PL-PFC. Behavioral tests with optogenetic stimulation in the PFC were done 2 weeks after viral injection. Tests with stimulation in the NAc core were done 4 weeks after injection to ensure optimal expression of opsins.
Prior to behavioral tests, optic fibers were connected to a 473 nm laser diode through an FC/PC adapter (Shanghai Dream Lasers, Shanghai). Laser intensity was measured with a power meter (Thorlabs, Newton, NJ, USA) prior to behavior testing. The laser was delivered using a TTL pulse-generating box (Tucker-Davis Technologies, Alachua, FL, USA). A laser protocol that included alternating light-on and light-off epochs for 30 s each was provided for the duration of the mechanical allodynia test, Hargreaves’ test, and CPP test. Within the light-on epoch, the laser was pulsed at 20 Hz with 10 ms pulse length.