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Cell cycle detection kit

Manufactured by Nanjing Jiancheng
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Sourced in China

The Cell Cycle Detection Kit is a laboratory instrument designed to analyze the cell cycle distribution of a cell population. It provides quantitative information on the percentage of cells in different phases of the cell cycle, such as G0/G1, S, and G2/M phases. The kit utilizes flow cytometry technology to measure the DNA content of individual cells, which is a key indicator of the cell cycle stage.

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Lab products found in correlation

Trichloroacetic acid tca, by Merck Group (1 mentions) Streptavidin xl665, by PerkinElmer (1 mentions) Trizma base, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Annexin 5 fitc pi apoptosis kit, by Keygen Biotech (1 mentions) Oligomycin, by Merck Group (1 mentions) Xf calibrant solution, by Agilent Technologies (1 mentions) Rna extraction kit, by Takara Bio (1 mentions) Fetal bovine serum (fbs), by Natocor (1 mentions) L carnosine, by Merck Group (1 mentions) Xf assay medium, by Agilent Technologies (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Sybr premix ex taq, by Takara Bio (1 mentions) Rotenone, by Merck Group (1 mentions) Atp assay kit, by Beyotime (1 mentions)

3 protocols using cell cycle detection kit

1

Cell Cycle Analysis by Flow Cytometry

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The cell cycle was analyzed using a cell cycle detection kit following the manufacturer’s protocols (Nanjing Jiancheng Bioengineering Institute). Cells were plated in six-well plates at a density of 105 cells/well in 2 ml medium. Cells were collected and fixed in 70% ethanol at −20 °C overnight. After centrifugation at 400 × g for 5 min, cells were suspended in 100 μl RNase solution and incubated at 37 °C for 30 min. Then 400 μl PI solution was added and incubated for another 30 min at 4 °C. The cell cycle was analyzed using flow cytometry.
Li Y., Qin Y., Yang C., Zhang H., Li Y., Wu B., Huang J., Zhou X., Huang B., Yang K, & Wu G. (2017). Cardamonin induces ROS-mediated G2/M phase arrest and apoptosis through inhibition of NF-κB pathway in nasopharyngeal carcinoma. Cell Death & Disease, 8(8), e3024-.
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2

Palbociclib Bioassay Protocol

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Palbociclib (S80974, purity 99%) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). CDK2/CycA2 (04–102), CDK4/CyclinD3 (04–105), and CDK6/CyclinD3 (04–107) were all purchased from Carna Biosciences, Inc. (Kobe, Japanese). Streptavidin-XL665 was obtained from Cisbio Bioassays (France). Annexin V-FITC/PI Apoptosis Kit was purchased from KeyGEN BioTECH (Nanjing, China). Cell cycle detection kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Dimethyl sulfoxide (DMSO) was purchased from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). Sulforhodamine B (SRB), Trichloroacetic acid (TCA), and Trizma® base were all obtained from Sigma-Aldrich (St. Louis, MO).
Wang S., Han S., Cheng W., Miao R., Li S., Tian X, & Kan Q. (2022). Design, Synthesis, and Biological Evaluation of 2-Anilino-4-Triazolpyrimidine Derivatives as CDK4/HDACs Inhibitors. Drug Design, Development and Therapy, 16, 1083-1097.
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3

Mitochondrial Bioenergetics and Cell Viability

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L-Carnosine, rotenone, carbonyl cyanide ptrifluoromethoxyphenyl-hydrazone (FCCP),
and Oligomycin were from Sigma (St Louis, MO). Dulbecco’s modified Eagle’s
medium (DMEM) was from Hyclone (USA). Fetal bovine serum (FBS) was from NATOCOR
(Argentina). Annexin V-FITC/PI apoptosis detection kit was from Lianke
Biotechnology Co, Ltd. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetra-zolium
bromide (MTT), BCA protein assay kit, and ATP assay kit were from Beyotime
Institute of Biotechnology (Nanjing, China). XF assay medium and XF calibrant
solution were obtained from Seahorse Bioscience (USA). RNA Extraction Kit,
PrimeScript RT reagent kit, and SYBR Premix Ex Taq were from TakaRa
Biotechnology (Dalian) Co, Ltd (Dalian, China). Cell cycle detection kit and
lactic acid assay kit were from Jiancheng Bioengineering Institute (Nanjing,
China).
Bao Y., Ding S., Cheng J., Liu Y., Wang B., Xu H., Shen Y, & Lyu J. (2016). Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integrative Cancer Therapies, 17(1), 80-91.
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