Anti cd19 antibody

Peripheral blood samples were collected from all study participants and the lymphocytes were isolated with lymphocyte separation liquid. A magnetic bead separation system (Miltenyi Biotec GmbH) was used to sort CD19⁺ B cells with anti-CD19⁺ antibody (cat. no. 130-113-733; 1:50; Miltenyi Biotec GmbH). Sorted CD19⁺ B cells were cultured for scale-up.

Peripheral blood mononuclear cells (PBMCs) were isolated by gradient centrifugation over Histopaque-1077 (density: 1.077 g/ml, Sigma-Aldrich) at 2,000 rpm for 20 min at 25℃. Lymphocytes were isolated from the spleens as previously described (28 (link)). The lungs were perfused with ice-cold phosphate-buffered saline (PBS) before removal for lymphocyte isolation. Lymphocytes from the lungs were isolated by using Percoll solution (Sigma-Aldrich) as previously described (27 (link)). B cells were separated from the spleen of naïve or LCMV CL13-infected mice using magnetic bead labeled with anti-CD19 antibody (Miltenyi Biotec). Purified B cells are >95% CD19 positive.