Pe conjugated anti cd95 antibody

Example 15

CD95 Induction Independent of Fc Receptor Interaction

Ramos cells were incubated overnight at 37° C., 6% CO₂ with various concentrations of the human anti-CD40 mAb's, (both whole IgG and F(ab′)2 fragments as indicated). The next day they were washed once with PBA and stained with PE-conjugated anti-CD95 antibody (Becton Dickinson) for 20 minutes at room temperature with shaking. The excess labeled antibody was washed off and the samples read a FACSCanto II™ instrument (BD Biosciences, NJ, USA). Results are shown in FIG. 17. These data indicate that Fc receptor interactions are not required by 3G5 to induce the expression of CD95 on the CD40+ human lymphoblastoid line Ramos.

Example 15

CD95 Induction Independent of Fc Receptor Interaction

Ramos cells were incubated overnight at 37° C., 6% CO₂ with various concentrations of the human anti-CD40 mAb's, (both whole IgG and F(ab′)2 fragments as indicated). The next day they were washed once with PBA and stained with PE-conjugated anti-CD95 antibody (Becton Dickinson) for 20 minutes at room temperature with shaking. The excess labeled antibody was washed off and the samples read a FACSCanto II™ instrument (BD Biosciences, NJ, USA). Results are shown in FIG. 17. These data indicate that Fc receptor interactions are not required by 3G5 to induce the expression of CD95 on the CD40+ human lymphoblastoid line Ramos.

Example 15

CD95 Induction Independent of Fc Receptor Interaction

Ramos cells were incubated overnight at 37° C., 6% CO₂ with various concentrations of the human anti-CD40 mAb's, (both whole IgG and F(ab′)2 fragments as indicated). The next day they were washed once with PBA and stained with PE-conjugated anti-CD95 antibody (Becton Dickinson) for 20 minutes at room temperature with shaking. The excess labeled antibody was washed off and the samples read a FACSCanto II™ instrument (BD Biosciences, NJ, USA). Results are shown in FIG. 17. These data indicate that Fc receptor interactions are not required by 3G5 to induce the expression of CD95 on the CD40+ human lymphoblastoid line Ramos.