Oci aml2 cells

The 293T cells were purchased from American Type Culture Collection (catalog no. CRL-3216) and cultured in Dulbecco’s modified Eagle medium (DMEM). The leukemia cell lines MOLM13 and OCI-AML2 cells (from M. Goodell) were cultured in RPMI-1640 or minimum essential medium α, all supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Pen/Strep) (all reagents from Gibco). Cells were cultured in a humidified atmosphere containing 5% CO₂ at 37°C. All cell lines tested negative for mycoplasma using a PCR-based method.

OCI-AML3 cells (German Collection of Microorganisms and Cell Cultures [DSMZ], Braunschweig, Germany) were cultured in RPMI 1640 medium (#01-100-1ACS; Biological Industries; Kibbutz Beit-Haemek, Israel) containing 20% fetal bovine serum (FBS) (#10270-106; Gibco, Waltham, MA, USA). OCI-AML2 cells (DSMZ) were cultured in MEM-alpha medium (#12561-056, Gibco) containing 20% FBS. U937 (American Type Culture Collection [ATCC], Manassas, VA, USA), HL-60 (ATCC), and THP-1 (ATCC) cells were cultured in RPMI 1640 medium containing 10% FBS. KG-1a cells (ATCC) were cultured in IMDM (#SH30228.01; Hyclone, Logan, UT, USA) supplemented with 20% FBS. Human mesenchymal stem cells (hMSCs, China National Collection of Authentic Cell Cultures [NCACC]) were cultured in a mesenchymal stem cell medium (#7501; ScienCell, Carlsbad, CA, USA) containing 5% FBS. The 293T cell line (ATCC) was cultured in DMEM (#SH30243.01, Hyclone) supplemented with 10% FBS. IMS-M2 cell line was a kind gift from Dr. Li He at Zhongnan Hospital of Wuhan University (Wuhan, China) and cultured in RPMI 1640 medium containing 20% FBS. All cell lines were cultured at 37 °C in a humidified chamber in the presence of 5% CO₂. Penicillin/streptomycin (#SV30010, Hyclone) was added to all cell cultures, except for the medium used for plasmid/siRNA transfection.