In all, 3.5 µL sample was applied onto a freshly glow discharged (70 s, 5 W, Plasma Cleaner Glow Discharge) 300 mesh R 1.2/1.3 holey carbon Film (Quantifoil) Cu grid30 . Excess sample was blotted away using Whatman No. 1 filter paper with blot force 4, blot time 3–6 s and wait time 5 s then vitrified by plunge freezing into liquid ethane cooled by liquid nitrogen using vitrobot Mark IV (Thermo Fisher Scientific, USA).
R 1.2 1 3 holey carbon film
Manufactured by Quantifoil
The R 1.2/1.3 holey carbon Film is a specialized lab equipment produced by Quantifoil. It is a thin carbon film with a regular pattern of precisely distributed holes. The film serves as a support structure for samples in various microscopy and analytical techniques.
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Lab products found in correlation
2 protocols using r 1.2 1 3 holey carbon film
1
Vitrification of Biological Samples
2
Graphene/Silica Transfer for TEM Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Graphene/silica
films were transferred
from the copper substrate by etching in 15% nitric acid and fishing
the floating film (without polymer support) onto commercial TEM grids
(Quantifoil R1.2/1.3 holey carbon film on Au 200 mesh). Afterward,
the specimen was rinsed in distilled water dried in dry nitrogen.
Prior to TEM, the samples were heated in air to 200 °C for 10
min.
films were transferred
from the copper substrate by etching in 15% nitric acid and fishing
the floating film (without polymer support) onto commercial TEM grids
(Quantifoil R1.2/1.3 holey carbon film on Au 200 mesh). Afterward,
the specimen was rinsed in distilled water dried in dry nitrogen.
Prior to TEM, the samples were heated in air to 200 °C for 10
min.
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